Cloning and characterization of the bipA gene encoding ER chaperone BiP from Aspergillus oryzae
Cloning and characterization of the bipA gene encoding ER chaperone BiP from Aspergillus oryzae
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DOI：
10.1016/s1389-1723(00)87661-6
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发表时间：
1999-11-01
影响因子：
2.8
通讯作者：
Kitamoto, K
中科院分区：
文献类型：
--
作者：
Kasuya, T;Nakajima, H;Kitamoto, K
我们描述了来自丝状真菌米曲霉的内质网定位伴侣 (BiP) 编码基因的克隆和表征。 BiP编码基因称为bipA，具有3个内含子，编码672个氨基酸的蛋白质，与多种BiP样蛋白质具有高度同源性。在酿酒酵母 KAR2 启动子中发现的类似于热休克元件 (HSE) 和未折叠蛋白反应 (UPR) 元件的序列存在于 bipA 基因的 5' no 编码区中。热休克或衣霉素处理可增加 bipA 基因的转录。 bipA cDNA 的表达部分补充了酿酒酵母 kar2 突变等位基因的温度敏感生长。这些结果表明bipA基因产物在米曲霉中发挥BiP的作用。
We describe the cloning and characterization of the ER localized chaperone (BiP) encoding gene from the filamentous fungus Asgergillus oryzae. The BiP encoding gene, designated bipA, has three introns and encodes a protein with 672 amino acids, which has a high homology with various BiP-like proteins. Sequences resembling heat shock elements (HSEs) and unfolded protein response (UPR) elements, as found in the Saccharomyces cerevisiae KAR2 promoter, are present in the 5' no coding region of the bipA gene. Transcription of the bipA gene was increased by heat shock or tunicamycin treatment. Expression of bipA cDNA partially complemented the temperature-sensitive growth of kar2 mutant alleles of S. cerevisiae. These results indicate that the bipA gene product plays a role as BiP in A. oryzae.