Identification of N7-(1-carboxyethyl)-arginine, a novel posttranslational protein modification of arginine formed at high hydrostatic pressure
Identification of N7-(1-carboxyethyl)-arginine, a novel posttranslational protein modification of arginine formed at high hydrostatic pressure
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DOI：
10.1196/annals.1333.006
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发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Schieberle, P
中科院分区：
文献类型：
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作者：
Alt, N;Schieberle, P
将N-α-乙酰精氨酸（0.1 mol/L）和葡萄糖（0.3 mol/L）在0.5 mol/L 3-[N-吗啉代]丙磺酸（5 mL; pH 7.0）中的水溶液在110 ℃下孵育2 h，同时施加高静水压（HHP）。酶促脱乙酰化后，通过离子交换色谱法分离形成的精氨酸修饰。除N-5-（4，5-二氢-4-甲基-5-氧代-1H-咪唑-2-基）-L-鸟氨酸外，还基于NMR和LC/MS测量和合成鉴定了新的精氨酸修饰物N7-（1-羧乙基）-精氨酸，特别是在600 MPa压力下反应的样品中。与在常压下处理的样品相比，两种化合物在增加的压力下以更高的量形成。
An aqueous solution of N-alpha-acetylarginine (0.1 mol/L) and glucose (0.3 mol/L) in 0.5 mol/L 3-[N-morpholinolpropanesulfonic acid (5 mL; pH 7.0) was incubated at 110 degrees C for 2 h with application of high hydrostatic pressure (HHP). After enzymic deacetylation, the arginine modifications formed were separated by ion exchange chromatography. Besides N-5-(4,5-dihydro-4-methyl-5-oxo-1H-imidazol-2-yl)-L-ornithine, the novel arginine modification N7-(1-carboxyethyl)-arginine was identified based on NMR and LC/MS measurements and synthesis-in particular, in samples reacted at a pressure of 600 MPa. As compared to samples treated at normal pressure, both compounds were formed in higher amounts at increased pressure.