Kinesin-5 Eg5 mediates centrosome separation to control spindle assembly in spermatocytes
Kinesin-5 Eg5 mediates centrosome separation to control spindle assembly in spermatocytes
复制标题
DOI：
10.1007/s00412-022-00772-5
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Wei Ya-Lan
中科院分区：
文献类型：
--
作者：
She Zhen-Yu;Zhong Ning;Wei Ya-Lan
Timely and accurate centrosome separation is critical for bipolar spindle organization and faithful chromosome segregation during cell division. Kinesin-5 Eg5 is essential for centrosome separation and spindle organization in somatic cells; however, the detailed functions and mechanisms of Eg5 in spermatocytes remain unclear. In this study, we show that Eg5 proteins are located at spindle microtubules and centrosomes in spermatocytes both in vivo and in vitro. We reveal that the spermatocytes are arrested at metaphase I in seminiferous tubules after Eg5 inhibition. Eg5 ablation results in cell cycle arrest, the formation of monopolar spindle, and chromosome misalignment in cultured GC-2 spd cells. Importantly, we find that the long-term inhibition of Eg5 results in an increased number of centrosomes and chromosomal instability in spermatocytes. Our findings indicate that Eg5 mediates centrosome separation to control spindle assembly and chromosome alignment in spermatocytes, which finally contribute to chromosome stability and faithful cell division of the spermatocytes.
