Response of Anchorage-Dependent Animal Cells to Fluid Shear Stress

最近，人们对培养依赖于锚定的动物细胞以生产具有重要医学意义的生物化学物质以及将这些细胞用于人工器官的可能性越来越感兴趣。这些细胞与体外环境的相互作用还知之甚少。这项研究的目的是检查依赖锚定的动物细胞对流体剪应力的反应。一个平行的平板流动通道将被建造和测试，以使电池暴露在明确定义的层流流场中。细胞从表面分离以及细胞行为和代谢的非致命性变化(如渗透性增加和形态变化)和蛋白质分泌将作为所施加剪应力的大小和持续时间的函数来检查。将通过研究生长在各种天然和合成表面上的细胞对剪应力的反应来检验表面对细胞分离和其他细胞行为变化的影响。

Recently, there has been increased interest in growing anchorage-dependent animal cells for the production of medically important biochemicals as well as for the possible use of these cells in artificial organs. The interaction of these cells with the in-vitro environment is poorly understood. The objective of this study is to examine the response of anchorage-dependent animal cells to fluid shear stresses. A parallel plate flow channel will be constructed and tested to expose the cells to a well-defined laminar flow field. Cell detachment from the surface as well as nonlethal changes in cell behavior and metabolism (such as increased permeability and morphological changes) and protein secretion will be examined as a function of the magnitude and duration of the applied shear stress. The effect of the surface on cell detachment and other changes in cell behavior will be examined by studying the response to shear stress of cells grown on a variety of natural and synthetic surfaces.