Dynein ATPase in Intracellular Motility

细胞内运动中的动力蛋白 ATP 酶

• 批准号: 8700366
• 负责人: Melanie Pratt
• 金额: $ 23.1万
• 依托单位: University of Miami School of Medicine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-11-01 至 1991-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8700366&HistoricalAwards=false
• 关键词: Dynein; ATPase; Intracellular; Motility

Several forms of intracellular transport, or movement, which are essential for normal cell growth and function depend on linear intracellular elements called microtubules. One example is chromosome movement during cell division; another is the transporrt of neurotransmitter-containing vesicles from one end of a nerve cell to the other. Two molecules have been described which serve as "molecular motors;" i.e., which are able to convert the chemical energy of ATP into mechanical force associated with the microtubules. One is called kinesin, and the other is called dynein. The objective of this proposal is to test the idea that an unusual form of dynein found in sea urchin eggs is the motor for microtubule-associated motility in this system. The specific goals of the project are: first, to obtain specific antibodies against the sea urchin egg dynein and use these to localize and quantitate dynein during embryonic development; then, to obtain a DNA clone for the sea urchin dynein using recombinant DNA techniques, and to use this clone as a probe to determine whether there are multiple, and possibly even tissue and stage-specific dynein genes. The anticipated results will add new and important information regarding the molecular basis of microtubule-based motility.

几种形式的细胞内运输或运动， 对正常细胞生长和功能至关重要， 称为微管的线性细胞内元件。 一个示例 一个是细胞分裂过程中的染色体运动;另一个是 含神经递质囊泡从一端转运 一个神经细胞到另一个。 有两种分子被描述为 其用作“分子马达”;即，其能够 将ATP的化学能转化为机械力 与微管相关的。 一种叫做驱动蛋白， 另一种叫做动力蛋白。 本提案的目的 是为了验证在海洋中发现的一种不寻常的动力蛋白 海胆卵是微管相关运动的发动机， 这系统. 该项目的具体目标是：第一， 获得针对海胆卵动力蛋白的特异性抗体， 用这些来定位和定量动力蛋白在胚胎 然后，为了获得海胆的DNA克隆， 利用重组DNA技术， 一个探测器，以确定是否有多个， 甚至组织和阶段特异性动力蛋白基因。 预期 结果将增加新的和重要的信息， 微管运动的分子基础