Assembly and Dissassembly of Animal Viruses

PI最近发现，病毒样外壳可以 在体外组装，使用获得的14S五聚体亚基 自然产生的空衣壳（即原衣壳）。 该系统为设计一种体外 能够形成脊髓灰质炎病毒粒子的反应混合物。 主要 本提案的目的是建立这样一种反应， 混合物，并将其用作纯化和分离 存在于Hela中的病毒特异性“形态生成”因子 负责形成原衣壳（和病毒）的细胞 14S材料中的颗粒）。 实验将 目的：（1）确定其性质（2）确定 它的作用方式，和3）定量所需的量， 支持体外病毒组装系统。 还计划进行另外两个系列的实验。 第一 旨在描述 病毒体和单克隆抗体之间的差异 病毒衣壳表位。 第二个问题涉及 脊髓灰质炎病毒“内部”起始位点的意义 核糖核酸 整个项目将大大有助于 我们对脊髓灰质炎病毒和其他病毒的蛋白质 合成和加工小核糖核酸病毒。

The PI has recently found that virus-like shells can be assembled in vitro, using 14S pentameric subunits obtained from naturally-occuring empty capsids (i.e. procapsids). This system provides a basis for designing an in vitro reaction mixture capable of forming poliovirions. The main objective of this proposal is to establish such a reaction mixture and to use it as an assay to purify and isolate the viral-specific "morphopoietic" factor(s) present in Hela cells which is responsible for forming procapsids (and virus particles) from 14S material in vivo. Experiments will be conducted to: 1) identify its nature 2) ascertain its mode of action, and 3) quantitate the amount needed to support the in vitro virus assembly system. Two series of other experiments are also planned. The first is aimed at characterizing the nature of the interactions between the virions and monoclonal antibodies to different viral capsid epitopes. The second deals with the significance of the "internal" initiation site in polioviral RNA. The project as a whole will contribute substantially to our understanding of the way proteins of poliovirus and other picornaviruses are synthesized and processed.