The Molecular Biology of Polydnavirus Gene Organization and Expression in Campoletis Sonorensis and Heliothis Virescens

多DNA病毒基因组织和在Campoletis Sonorensis和Heliothis Virescens中表达的分子生物学

• 批准号: 8701918
• 负责人: Max Summers
• 金额: $ 24万
• 依托单位: Texas A&M Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8701918&HistoricalAwards=false
• 关键词: Molecular; Biology; Polydnavirus; Gene; Organization

The successful parasitism by a parasitic insect wasp of its host insect is dependent upon the transfer and expression of a polydnavirus along with the parasite's egg during oviposition. The virus and its life cycle are unique in two major aspects: 1) it is a DNA animal virus with a complex multipartite genome consisting of at least 28 doublestranded, covalaently closed DNAs; 2) the virus has an apparent symbiotic relationship with the wasp insect and causes certain changes in the parasitized host effectively compromising the host's cellular immune defenses to favor the successful development of the parasite. The parasitic Campoletis sonorensis wasp, its virus (CsV), and the host insect, Heliothis virescens, is the most thoroughly studied parasite-virus-host system at the molecular level. Studies will be continued on mapping the genetic organization of the multipartite genome. This will be done by physical mapping and a mapping of the transcription products in the wasp insect and parasitized host. Once mapped those genes believed to be important factors interfering with the parasitized insect's cellular defense system will be cloned and studied for function and identity of viral encoded gene products. It is the ultimate goal of the research to identify the specific functions of the virus and mechanisms (target sites and effects) in the parasitized insect abrogating cellular immune response. This should lead to an elucidation of insect immunity defenses and how these may be manipulated for the control of insects of public health or agricultural importance, or for control of the ability of insects to transmit animal and plant pathogens.

寄生蜂寄生昆虫黄蜂对其寄主的成功寄生 昆虫依赖于一种基因的转移和表达， 多DNA病毒沿着寄生虫的卵。 该病毒及其生命周期在两个主要方面是独特的： 它是一种DNA动物病毒，具有复杂的多部分基因组， 由至少28个双链共价闭合的 （2）病毒具有明显的共生关系， 黄蜂昆虫，并导致某些变化，在寄生 有效地损害宿主的细胞免疫防御 以利于寄生虫的成功发育 的 寄生的Campoletissonorensis黄蜂，其病毒（CsV）， 宿主昆虫烟芽夜蛾是研究最彻底的昆虫 寄生虫-病毒-宿主系统。 研究将 将继续绘制 多分裂基因组 这将通过物理映射和 黄蜂昆虫中转录产物的定位， 寄生宿主 一旦绘制出那些被认为是 干扰被寄生昆虫的重要因素 细胞防御系统将被克隆并研究其功能 和病毒编码基因产物的身份。 这是最终的 研究的目的是确定 病毒和机制（靶点和效应） 寄生昆虫消除细胞免疫反应。 这 这将有助于阐明昆虫的免疫防御机制， 这些可以被操纵用于控制公共场所的昆虫， 健康或农业的重要性，或控制的能力， 昆虫传播动植物病原体。