Characterization of Altered Flagellin Genes from Color Variants of Serratia Marcescens

粘质沙雷氏菌颜色变体中改变的鞭毛蛋白基因的表征

• 批准号: 8701987
• 负责人: Rasika Harshey
• 金额: $ 19.49万
• 依托单位: Scripps Clinic and Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1990-01-01
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8701987&HistoricalAwards=false
• 关键词: Characterization; Altered; Flagellin; Genes; Color

One of the most striking properties of the pigmented bacterium Serratia marcescens is the phenomenon of color instability. Different strains differ greatly both in color and in the frequency with which they produce color variants. Within a strain, the variations occur at constant rates and are reversible. The high degree of genetic instability is reminiscent of DNA rearrangements such as transpositions, deletions or inversions which are found to occur during various biological processes. During an investigation of this phenomenon Dr. Harshey observed that variation of a 39 kilodalton protein in S. marcescens strain 274, is closely associated with color variation. Using antibodies to this protein she identified it as being the bacterial flagellin. Neither color nor flagellin are produced at temperatures above 37 oC. Thus, pigment synthesis and flagellin synthesis appear to be co-regulated. We propose to isolate and characterize the gene(s) for Serratia flagellin.

色素细菌最显著的特性之一 粘质沙雷氏菌是颜色不稳定的现象。 不同的菌株在颜色和颜色上都有很大的差异。 它们产生颜色变体的频率。 内 应变，变化以恒定速率发生， 可逆的 基因的高度不稳定性 让人联想到DNA重排，如易位， 缺失或倒位，这些缺失或倒位被发现发生在各种 生物过程。 在对这一现象的调查中， S.粘质菌株 第274章与颜色有关 使用 她发现这种蛋白质的抗体 细菌鞭毛蛋白 无论是颜色还是鞭毛蛋白， 温度高于37 oC。 因此，色素合成和鞭毛蛋白 合成似乎是共调节。 我们建议隔离和 表征沙雷氏菌属鞭毛蛋白的基因。