Role of Myosin in Intestinal Brush Border Function

肌球蛋白在肠刷状缘功能中的作用

• 批准号: 8702154
• 负责人: Thomas Keller
• 金额: $ 19万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8702154&HistoricalAwards=false
• 关键词: Role; Myosin; Intestinal; Brush; Border

The role of myosin in the structure and function of brush borders isolated from intestinal and colonic epithelial cells will be investigated. In the brush border cytoskeleton, myosin is associated with two structural domains: one comprising the terminal web where it crosslinks the microvillus core bundles of actin filaments, and the other a contractile ring of actin filaments that surrounds the terminal web. Two subsets of myosin are differentially extractable from the brush border: one that is involved in terminal web contraction and one that is not. The aim of this research is to determine the molecular basis for the two subsets. The possiblity that more than one isozyme of myosin is expressed in the brush border, and the relationship between myosin isozymes and the subsets of myosin will be investigated. The localization of each of the subsets of myosin with respect to the two major structural domains of the terminal web will also be determined. Because the circumferential ring is organized long before the interroolet region during embryogenesis, the findings on myosin isozyme expression and subset localization will be related to the normal sequence of intestinal development. Finally, the stable structural association of one myosin subset indicates the likelihood that it interacts with more than actin in the cytoskeleton. Proteins that bind myosin in the brush border will be identified and localized. The results of this research may indicate that myosin plays more than one role in brush border function and that a specific myosin performs a specific function within a single cytoskeletal system. This adds new significance to the general observation that cytoskeletal proteins are frequently encoded by multigene families.

肌球蛋白在刷毛结构和功能中的作用 从肠和结肠上皮细胞分离的边界 将进行调查。在刷状缘细胞骨架中，肌球蛋白 与两个结构域相关：一个包含 末端网，在那里它交联微绒毛核心束， 另一个是肌动蛋白的收缩环 围绕末端网的细丝。 肌球蛋白的两个亚类 从画笔边缘提取的差异：一个， 一个参与了末端网的收缩，另一个则没有。 的 这项研究的目的是确定的分子基础， 两个子集。 肌球蛋白的一种以上同工酶 在画笔边框中表示， 将研究肌球蛋白同工酶和肌球蛋白亚类。 肌球蛋白的每个亚群相对于 终端网的两个主要结构域也将 测定因为圆周环组织得很长 在胚胎发育过程中， 对肌球蛋白同工酶表达和亚群定位的影响 与肠道发育的正常顺序有关。 最后，一个肌球蛋白亚群的稳定结构关联 表明它与肌动蛋白以外的物质相互作用 在细胞骨架中。 在刷子中结合肌球蛋白的蛋白质 边界将被识别和定位。 的结果 研究可能表明，肌球蛋白在 刷状缘功能，一个特定的肌球蛋白执行 在单个细胞骨架系统内的特定功能。 这增加 新的意义的一般观察，细胞骨架 蛋白质通常由多基因家族编码。