RUI: Molecular Aspects of Effector Binding to Phosphoenolpyruvate Carboxylase from Crassula argentea

RUI：效应子与银景天磷酸烯醇丙酮酸羧化酶结合的分子方面

• 批准号: 8707046
• 负责人: Scott Grover
• 金额: --
• 依托单位: California State University-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-15 至 1991-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8707046&HistoricalAwards=false
• 关键词: RUI; Molecular; Aspects; Effector; Binding

Dr. Grover is studying a key regulatory enzyme of plants adapted to a high temperature, low moisture environment. The CAM plants, commonly known as succulents, absorb carbon dioxide through stomata in their leaves only at night where temperatures are low and water loss through these leaf openings is minimized. Reactions of carbon fixation and metabolism are regulated on a diurnal basis and the enzyme PEP carboxylase plays a key role here. With the assistance of undergraduate students, Dr. Grover will determine how certain known "regulatory ligands" bind to and control the activity of this enzyme. He will probe the physical nature of the binding sites with chemical modification and labeling. This study will likely lead to knowledge of new regulatory mechanisms for enzymes of photosynthesis, an important payoff.

格罗弗博士正在研究植物适应高温、低湿度环境的一种关键调节酶。 CAM 植物（通常称为多肉植物）仅在夜间通过叶子中的气孔吸收二氧化碳，此时温度较低，通过叶子开口损失的水分也最少。碳固定和代谢反应每天都会受到调节，PEP 羧化酶在此发挥关键作用。在本科生的帮助下，格罗弗博士将确定某些已知的“调节配体”如何与这种酶结合并控制其活性。他将通过化学修饰和标记来探索结合位点的物理性质。这项研究可能会带来对光合作用酶的新调控机制的了解，这是一个重要的回报。