PostDoctoral Research Fellowships in Plant Biology

植物生物学博士后研究奖学金

• 批准号: 8710581
• 负责人: Marc Orbach
• 金额: $ 5.34万
• 依托单位: Individual Award
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-10-01 至 1989-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8710581&HistoricalAwards=false
• 关键词: PostDoctoral; Research; Fellowships; Plant; Biology

The ability of plants to resist pathogenic invasion by fungi often involves interactions between specific host and pathogen genotypes. Genes from the bean pathogen, Glomerella cingulata, that are responsible for specific interactions with Phaseolus vulgaris will be isolated. Experiments with the cloned genes will address the mechanisms responsible for specific recognition between host and pathogen. The genes will be used to test whether their expression is induced by exposure to plants; if so, factors responsible for induction will be identified. Antibodies made against proteins encoded by these genes will be used to detect their presence and cellular localization. Experiments will be performed to determine if these proteins are the primary signals for recognition by resistant hosts, or if they are cell- wall degrading enzymes that release specific elicitors that are recognized by the host. If the fungal proteins are the signals, they will be used to detect the plant receptors. Knowledge of how pathogens and their hosts interact on both the genetic and metabolic level is of basic interest but the practical considerations are also immense. Crops are lost each year to various diseases and only by understanding the basis of disease can rational preventatives be designed.

植物抵抗病原真菌入侵的能力往往涉及到特定寄主和病原基因之间的相互作用。来自菜豆病原菌Glmerella cingulata的基因将被分离出来，这些基因负责与菜豆的特定相互作用。用克隆的基因进行的实验将解决宿主和病原体之间负责特定识别的机制。这些基因将被用来测试它们的表达是否由暴露在植物中诱导；如果是，将确定诱导因素。针对这些基因编码的蛋白质产生的抗体将被用来检测它们的存在和细胞定位。将进行实验，以确定这些蛋白质是耐药宿主识别的主要信号，还是释放宿主识别的特定激发子的细胞壁降解酶。如果真菌蛋白质是信号，它们将被用来检测植物受体。了解病原体及其宿主如何在遗传和代谢水平上相互作用是基本的兴趣，但实际考虑也是巨大的。农作物每年都会因各种疾病而损失，只有了解了疾病的基础，才能设计出合理的预防措施。