PostDoctoral Research Fellowships in Plant Biology

拟议的研究使用现有的舌特异性 突变体来研究极化细胞分裂是如何被调节的。的 这项研究的目的是检验假设， 细胞骨架在细胞的传递中起着结构性的作用。 指导细胞分裂的遗传信息。 的 假设将被测试表征细胞骨架 正常和舌特异性突变体的变化， 完整和切片叶组织的免疫细胞化学。 叶原基的显微操作将测试 叶舌的测定和抑制剂实验将测试 特定细胞骨架成分的作用。 时 通过放射自显影术确定的DNA合成将放置在 在细胞周期的背景下细胞骨架的变化。 以来 叶舌的划分是精确的和可预测的，比较研究 将提供一种方法来分离特定的 控制点划分。 这些结果将澄清 细胞骨架参与的过程 在分裂过程中建立细胞极性。 为了完全理解机械事件 这导致了一个有序的程序在开发中， 生物体，有必要使用遗传学，细胞， 生物学、生物化学等。然而， 几种专业知识的结合不是一个人就能得到的。 实验室和组合的方法不使用。 这 该项目汇集了遗传学和细胞生物学， 重大发展问题。 如果细胞骨架 作为细胞中遗传信息的管道， 这项研究的影响将体现在各个方面， 生物学，而不仅仅是植物生物学。

The proposed study uses the available ligule-specific mutants to ask how polarized cell divisions are regulated. The goal of the study is to test the hypothesis that the cytoskeleton plays a structural role in the transmission of the genetic information that directs cell division. The hypothesis will be tested characterizing the cytoskeletal changes of normal and ligule-specific mutants using immunocytochemistry of intact and sectioned leaf tissue. Micromanipulation of leaf primordia will test for the time of determination of the ligule, and inhibitor experiments will test for the role of specific cytoskeletal components. The time of DNA synthesis, as determined by autoradiography, will place the cytoskeletal changes within the context of the cell cycle. Since ligule division is precise and predictable, a comparative study with mutant plants will provide a means to isolate specific control points during division. These results will clarify the process by which the cytoskeleton participates in the establishment of cell polarity during division. In order to completely understand the mechanistic events which result in an ordered program in the developing organism, it is necessary to use the techniques of genetics, cell biology, biochemistry, etc. Too often, however, the several binds of expertise are not available to a single laboratory and the combined approach is not used. This project brings together genetics and cell biology to ask a significant developmental question. If the cytoskeleton is implicated as a conduit of genetic information in the cell by this study, the impact will be felt in all aspects of biology, not just the area of plant biology.