PostDoctoral Research Fellowship In Plant Biology

植物生物学博士后研究奖学金

• 批准号: 8710625
• 负责人: Christopher Hogan
• 金额: $ 7.95万
• 依托单位: Individual Award
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-11-01 至 1990-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8710625&HistoricalAwards=false
• 关键词: PostDoctoral; Research; Fellowship; Plant; Biology

The mechanisms involved in mitotic movements are central to our knowledge of cell biology yet remain poorly understood. Understanding the regulation of these mechanisms is crucial to development of an integrated model of cell division and cellular motility in general. Recently a model system has been developed where anaphase B (spindle elongation) can be studied in vitro using isolated, functional spindles from a diatom. Using this system it was found that spindle elongation in vitro requires a highly phosphorylated environment. Several phosphorylated proteins associated with the microtubule overlap zone were also identified. Protein phosphorylation thus may play a key role in the regulation of mitosis. The identification and characterization of the protein kinase(s) involved is proposed. An in vitro quantitative assay will be developed whereby phosphorylation levels of overlap zone proteins can be evaluated. Phosphorylated overlap zone proteins may be integral to spindle motor function. Also proposed is an immuno-analysis of these proteins. A monoclonal antibody library will be raised against phosphorylated overlap zone proteins. Any antibodies found to inhibit anaphase B will be used to purify the antigen for in vitro characterization. Antibodies also will be tried on higher plant cells to see if these proteins are common to the higher plant mitotic machinery as well. Mitosis is central to genetic inheritance in eukaryotic cells. Knowledge of the molecular mechanisms involved with the ordered movement of chomosomes is essential to any understanding of the mechanics of cell division as well as the transmission of genetic information. The results of this study should greatly enhance the minimal understanding we now have.

有丝分裂运动中涉及的机制是核心， 我们对细胞生物学的了解仍然很少。 了解这些机制的调节对于 细胞分裂和细胞分裂的综合模型的开发 一般的运动。 最近开发了一个模型系统 其中可以在体外研究后期B（纺锤体伸长 利用硅藻中分离的功能性纺锤体。 使用此 系统，发现体外纺锤体伸长需要一个 高度磷酸化的环境。 几个磷酸化 与微管重叠区相关的蛋白质也 鉴定 因此，蛋白质磷酸化可能在以下方面发挥关键作用： 有丝分裂的调节。 确定和 所涉及的蛋白激酶的表征是 提出了 将开发一种体外定量测定方法 由此重叠区蛋白的磷酸化水平可以 评估。 磷酸化重叠区蛋白可能是整合的 主轴电机功能。 还提出了一个 这些蛋白质的免疫分析。 单克隆抗体 将针对磷酸化重叠区产生文库 proteins. 任何发现的抑制后期B的抗体将被 用于纯化抗原以进行体外表征。 抗体也将在高等植物细胞上进行试验，看看是否 这些蛋白质是高等植物有丝分裂 机器也是。 有丝分裂是真核生物遗传的核心 细胞 了解参与的分子机制， 染色体的有序运动对于任何 了解细胞分裂的机制以及 遗传信息的传递。 的结果 这项研究应该大大提高我们现在最起码的理解， 有.