NMR Studies of the Cytochrome c Peroxidase: Cytochrome c Redox Complex

细胞色素c过氧化物酶：细胞色素c复合物具有重要的生理作用，在远距离电子转移反应中起作用。在阐明该复合体功能的细节方面已经取得了重要进展。预计的工作将：1)阐明过氧化物酶氧化中间体和铁细胞色素c之间的电子转移速率；2)研究细胞色素c过氧化物酶的电子转移速率：与酵母细胞色素c同工酶和/或金枪鱼细胞色素c交联。将使用各种物理技术，以及分子图形建模。旨在比较可能的复杂几何形状及其对细胞色素c序列变化的依赖性。

The cytochrome c peroxidase:cytochrome c complex is physiologically important and functions in long distance electron transfer reactions. Important progress in elucidating the details of the functioning of this complex have been made. The projected work will: 1) elucidate the rate of electron transfer between the peroxidase oxidized intermediates and ferrocytochrome c and 2) study the rate of electron transfer in cytochrome c peroxidase:cross linked with yeast cytochrome c isozymes and/or tuna cytochrome c. Various physical techniques will be used, as well as molecular graphics modelling, designed to compare the possible complex geometries and their dependence on sequence variations in the cytochromes c.