Structure - Function Relationships in Group Transfer Reactions

这个项目的目标是提供明确的答案 一系列的结构性和机械性问题， 酶学 通过使用多种实验方法，博士。 Villafranca希望能更深入地了解 酶的催化作用，特别是在结构关系方面 来运作。 维拉弗兰卡博士将专注于酶的研究， 涉及不同类型的ATP基团转移反应。 首先，CTP合成酶将用 关于GMP合成酶和丙酮酸磷酸一些补充工作 二激酶 通过使用几种结构和动力学方法 他将研究的性质和相对自由能 催化反应的中心酶复合物， 这些酶。 酶的基团同一性探讨 以及中间体的形成和分解速率， 该酶促反应将提供一组数据， 对催化效率进行机械推导， 基团转移反应。

The goals of this project are to provide definitive answers to a range of structural and mechanistic questions in enzymology. By using multiple experimental approaches, Dr. Villafranca hopes to reach a deeper level of understanding of enzyme catalysis, especially in the relationship of structure to function. Dr. Villafranca will focus on the study of enzymes that involve different types of group transfer reactions with ATP. Primarily, the enzyme CTP synthetase will be studied with some additional work on GMP synthetase and pyruvate phosphate dikinase. By using several structural and kinetic approaches he will investigate the nature and relative free energies of the central enzyme complexes for the reactions catalyzed by these enzymes. Exploration of the identity of enzymic groups and the rate of formation and breakdown of intermediates in this enzymatic reaction will provide a set of data with which to make mechanistic deductions about the catalytic efficiency of group transfer reactions.