Structure - Function Relationships in Group Transfer Reactions

基团转移反应中的结构-功能关系

• 批准号: 8717646
• 负责人: Joseph Villafranca
• 金额: $ 20.86万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8717646&HistoricalAwards=false
• 关键词: Structure; Function; Relationships; Group; Transfer

The goals of this project are to provide definitive answers to a range of structural and mechanistic questions in enzymology. By using multiple experimental approaches, Dr. Villafranca hopes to reach a deeper level of understanding of enzyme catalysis, especially in the relationship of structure to function. Dr. Villafranca will focus on the study of enzymes that involve different types of group transfer reactions with ATP. Primarily, the enzyme CTP synthetase will be studied with some additional work on GMP synthetase and pyruvate phosphate dikinase. By using several structural and kinetic approaches he will investigate the nature and relative free energies of the central enzyme complexes for the reactions catalyzed by these enzymes. Exploration of the identity of enzymic groups and the rate of formation and breakdown of intermediates in this enzymatic reaction will provide a set of data with which to make mechanistic deductions about the catalytic efficiency of group transfer reactions.

这个项目的目标是为酶学中的一系列结构性和机械性问题提供明确的答案。通过使用多种实验方法，维拉夫兰卡博士希望对酶催化，特别是结构与功能的关系有更深层次的了解。维拉夫兰卡博士将专注于涉及与ATP发生不同类型的基团转移反应的酶的研究。首先，将对CTP合成酶进行研究，并对GMP合成酶和丙酮酸磷酸二激酶进行一些额外的研究。通过使用几种结构和动力学方法，他将研究中心酶复合体的性质和由这些酶催化的反应的相对自由能。探索这种酶反应中酶基团的同一性以及中间体的形成和分解速度，将为从机理上推断基团转移反应的催化效率提供一组数据。