Mapping Recombination Nodules on Synaptonemal Complexes of Lycopersicon Esculentum (Tomato)

该项目旨在通过定位减数分裂后期粗线期与联会复合体相关的重组根瘤的分布来分析番茄植物花药细胞中的交叉分布。这将使用由调查人员开发的低张爆裂技术来完成。然后，重组根瘤图谱将被转换为沿着每个联会复合体的重组物理图谱，与现有的基因连锁图谱相关联。随后，信息将被转移到粗线期染色体上。该项目应该提供准确的基因图谱，并对染色体交叉提供进一步的洞察。该项目有可能在细胞遗传学领域产生对植物育种者有用的重要信息。

The project proposed is addressed to an analysis of the crossover distribution in tomato plant anther cells by mapping the distribution of recombination nodules associated with the synaptonemal complexes at late pachytene stage of meiosis. This will be done using a hypotonic bursting technique developed by the Investigator. The recombination nodule map will then be converted to a physical map of recombination along each synaptonemal complex, correlated with available gene linkage maps. Subsequently the information will be transferred to pachytene chromosomes. The project should provide accurate mapping of genes and provide further insight into chromosome crossover. The project has the potential to yield important information in the area of cytogenetics that will be of use to plant breeders.