Structure-Function Relationships of Astral Mitosis

在有丝分裂后期分离染色体所需的力将被检验，重点放在这些力的位置、功能和结构基础上。将特别关注微管在B后期的作用(S)。一系列独特的实验将允许独立于纺锤力来研究细胞质力量，因为纺锤力将通过对真菌镰刀菌活的有丝分裂细胞进行的激光微束显微手术来选择性地中和。这项工作的结果将改进真菌中纺锤体和酯功能的概念，这可能也与包括动物在内的广泛有机体的有丝分裂有关。

The forces required to separate chromosomes at mitotic anaphase will be examined, with emphasis on the location, function, and structural basis of those forces. Special attention will be paid to the role(s) of microtubules during anaphase B. A unique series of experiments will allow the cytoplasmic forces to be studied independently of spindle forces, because the spindle forces will be selectively neutralized by laser microbeam microsurgery performed on living mitotic cells of the fungus Fusarium. The results of this work will lead to improved concepts of spindle and aster functions in fungi that may be relevant also to mitosis in a wide range of organisms, including animals.