Circadian Pacemaker Function: SCN Transplant Approach

昼夜节律起搏器功能：SCN 移植方法

• 批准号: 8719531
• 负责人: Patricia DeCoursey
• 金额: $ 27.61万
• 依托单位: University of South Carolina at Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-15 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8719531&HistoricalAwards=false
• 关键词: Circadian; Pacemaker; Function; SCN; Transplant

Regulation of circadian rhythmicity in mammals by a neural pacemaker, the suprachiasmatic nuclei (SCN), will be studied using a new transplant technique recently developed in our laboratory; neural transplants of 14-day embryonic SCN restore circadian rhythmicity in adult male hamster hosts previously rendered arrthymic by electrolyhtic SCN lesioning. The planned transplant experiments will address: specificity of SCN in restoring rhyhthmicity, role of age of fetal transplant tissue in graft survival and restoration of rhythmicity, effect of transplant location on restoration of rhythmicity, neural or humoral mode of SCN action, rate and pathway of SCN transplant re- enervation, and degree of optic nerve fiber re-enervation for photoentrainment. Locomotor activity will be used to monitor daily rhythmicity of intact hosts initially in constant conditions (DD), as well as after lesioning and transplantation and in a final light schedule of LD entrainment. Subsequently, animals will be sacrified, hypothalamic areas will be serially sectioned, and the tissue stained with histological and immunocytological methods to confirm viability of transplants, their normal neuropeptide expression, and completeness of host lesioning. SCN transplantation holds promise, in specific term, for elucidating SCN pacemaker mechanisms. More generally, the transplant paradigm may serve as a model for understanding neural mechanisms.

将使用我们实验室最近开发的新移植技术来研究神经起搏器视交叉上核（SCN）对哺乳动物昼夜节律的调节； 14天胚胎SCN的神经移植恢复了成年雄性仓鼠宿主的昼夜节律，该宿主先前因电解SCN损伤而出现节律失常。 计划的移植实验将讨论：SCN在恢复节律性方面的特异性、胎儿移植组织的年龄在移植物存活和节律性恢复中的作用、移植位置对节律性恢复的影响、SCN作用的神经或体液模式、SCN移植重新神经化的速率和途径，以及视神经纤维重新神经化的程度。 光夹带。 运动活动将用于监测完整宿主的日常节律性，最初在恒定条件下（DD），以及在损伤和移植后以及在LD夹带的最终光照时间表中。 随后，处死动物，连续切片下丘脑区域，并用组织学和免疫细胞学方法对组织进行染色，以确认移植物的活力、其正常的神经肽表达以及宿主病变的完整性。 具体而言，SCN 移植有望阐明 SCN 起搏机制。 更一般地说，移植范式可以作为理解神经机制的模型。