Transmitter Release During Synaptogenesis

突触发生期间的递质释放

• 批准号: 8719613
• 负责人: Steven Young
• 金额: $ 14.47万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-15 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8719613&HistoricalAwards=false
• 关键词: Transmitter; Release; During; Synaptogenesis

During development of the synapse, the nerve cells send out processes, the neurites, which grow out to contact the target cells. As part of the process of maturation of this initial contact into a functioning synapse, the nerve must start to release transmitter onto the target cell, and in addition, the nerve-target contact must be stable and continue to release transmitter. The experiments of this grant will focus on these two important areas of synaptic development: Identifying the process(es) which initiate the start of transmitter release at early nerve-target contacts and the mechanisms that contribute to the stability of this release. Cultured nerve and muscle cells of the frog Xenopus laevis will be used in combination with the patch clamp technique in order to achieve a high level of control over the geometry and resolution of the measured transmitter release. The major goal of these experiments is to enhance the understanding of the initiation and stabilization of transmitter release at developing synapses.

在突触的发育过程中，神经细胞发出 突起，即神经突，它生长出来接触目标， 细胞 作为这一初始成熟过程的一部分， 接触到一个功能性突触，神经必须开始 释放发射器到目标细胞上，此外， 神经-目标接触必须稳定并持续释放 发送器 这项赠款的实验将集中在这些方面 突触发育的两个重要领域： 启动发射机释放开始的过程 早期的神经靶点接触和机制， 这个版本的稳定性。 非洲爪蟾的培养神经和肌肉细胞将 与膜片钳技术结合使用， 实现对几何形状和分辨率的高水平控制 测量的发射器释放量。 这些实验的主要目标是提高 对发射机启动和稳定的理解 在发育中的突触释放。