Data Collection Instrumentation for Protein Crystallography

蛋白质晶体学数据采集仪器

• 批准号: 8719815
• 负责人: Gregory Petsko
• 金额: $ 25万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-05-01 至 1990-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8719815&HistoricalAwards=false
• 关键词: Data; Collection; Instrumentation; Protein; Crystallography

This proposal requests support to obtain a high-efficiency data collection instrument for protein X-ray crystallography. This instrument will be used for the solution of protein crystal structures, specifically those of penicillin acylase, D-amino acid transaminase, beta-keto thiolase, alanine racemase, and an anti-digoxin monoclonal antibody Fab fragment. It will also be used for studies of mutants and substrate complexes of L- aspartate amino transferase, triose phosphate isomerase, glucose isomerase, the serine proteases chymotrypsin and elastase, and iron superoxide dismutase.

这一建议要求得到支持，以获得高效率的 用于蛋白质X射线晶体学的数据收集仪器。 该仪器将用于蛋白质晶体的溶解 结构，特别是青霉素酰化酶，D-氨基 酸性转氨酶、β-酮硫解酶、丙氨酸消旋酶和 抗地高辛单克隆抗体Fab片段。还将 用于研究L- 天冬氨酸氨基转移酶，磷酸丙糖异构酶，葡萄糖 异构酶、丝氨酸蛋白酶胰凝乳蛋白酶和弹性蛋白酶，和 铁超氧化物歧化酶