Genetic Control of Drosophila Embryonic Pattern Formation

果蝇胚胎模式形成的遗传控制

• 批准号: 8801814
• 负责人: Sean Carroll
• 金额: $ 23.84万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8801814&HistoricalAwards=false
• 关键词: Genetic; Control; Drosophila; Embryonic; Pattern

The development of the metameric body plan of the insect Drosophila melanogaster has emerged as the principal model system for studying the genetic and molecular basis of embryonic pattern formation. The objectives of this proposal are to make a comprehensive study of the interactions between early-acting genes of the zygote that control subsequent gene expression and determine the fates of groups of cells. By using molecular probes (cloned DNA and antibodies) to study gene expression in wild-type and a great variety of mutant embryos, the genetic system that controls the time, place and level of pattern- regulating gene expression will be systematically dissected. Individual regulatory phenomena revealed by these previous studies may eventually be studied at the biochemical level in order to determine the mechanisms of gene regulation and function. The conceptual framework of development being revealed by studies in Drosophila relates to the fundamental aspects of any complex organism. The control of cell proliferation, gastrulation, germ layer differentiation, position-specific gene expression, and the number and differentiation of body parts and appendages are central themes to all complex animals. By exploiting the genetic approach available in Drosophila and combining it with advanced molecular and embryological techniques, it is likely that more will be known about the growth, development, and differentiation of the fruit fly than any other animal species. %%% The development of a complex animal from a simple fertilized egg is one of the most important problems in biology that remains to be solved. In Dr. Carroll's laboratory, he is studying the genes that control this process in the fruit fly, one of the best understood genetic model system for developmental studies. By studying these genes, and the protein molecules that they encode, he aims to understand how cells are organized into distinct groups to form the different tissues, organs, and regions of the animal's body.

昆虫同色异谱体平面的发展 黑腹果蝇已经成为主要的模型系统 用于研究胚胎模式的遗传和分子基础 阵这项建议的目的是， 对早期作用的药物和 控制随后基因表达的合子基因， 决定细胞群的命运。 利用分子 探针（克隆的DNA和抗体）研究基因表达， 野生型和各种各样的突变胚胎，遗传 控制时间、地点和模式级别的系统- 调节基因表达将被系统地剖析。 这些以往的个案所揭示的个别监管现象 研究最终可能会在生物化学水平上进行研究， 为了确定基因调控的机制， 功能 发展的概念框架正在显现 果蝇的研究涉及到 任何复杂的有机体。 控制细胞增殖， 原肠胚形成，胚层分化，位置特异性基因 表达，以及身体部位的数量和分化， 附肢是所有复杂动物的中心主题。 通过 利用果蝇的遗传学方法， 结合先进的分子和胚胎学技术 技术，很可能会有更多的了解， 生长、发育和分化的果蝇比 任何其他动物物种。 %%% 从简单的受精卵发育成复杂的动物 卵子是生物学中最重要的问题之一， 有待解决。 在卡罗尔博士的实验室里，他正在研究 果蝇中控制这一过程的基因， 了解发育研究的遗传模型系统。 通过 研究这些基因和它们编码的蛋白质分子， 他的目标是了解细胞是如何组织成不同的 不同的组织、器官和区域， 动物的身体。