RUI: Structrual Analysis of RNase P, An RNA Catalyst

RUI:RNA 催化剂 RNase P 的结构分析

基本信息

  • 批准号:
    8804218
  • 负责人:
  • 金额:
    $ 8.16万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    1988
  • 资助国家:
    美国
  • 起止时间:
    1988-09-01 至 1990-09-01
  • 项目状态:
    已结题

项目摘要

The dynamic capabilities of RNA have been fully realized with the discovery that RNA molecules can perform as catalysts in secondary reactions of the central dogma. The endonucleolytic activity of RNase P RNA on its substrate, precursor tRNA, was the second such activity to be described. It is proposed to continue the investigation on the structure of RNase P in order to further define the mechanism of catalysis and the intracellular structure of RNase P. Further it is proposed to extend the understanding of the phylogenetics of this RNA catalyst as well as to begin a search for other physiologically important small RNAs. The structural analysis of RNase P RNA will make use of comparative sequencing of RNase P RNAs from several microorganisms as well as chemical and enzymatic probes of structure at various temperatures and ionic conditions. The phylogenetics of RNase P will be explored by examining Deinococcus radiodurans, Micrococcus roseus, Clostridium sporogenes, Halobacter solanarium, and Thermoplasma acidophilum using a synthetic precursor tRNA as substrate. The intracellular conformation of the RNase P will be examined by the isolation of the activity under conditions that preserve macromolecular interactions and, alternatively, by asking what proteins bind to purified RNase P RNA. Other small RNAs isolated will be tested for evolutionary conservation (hence physiological importance). RNA has recently been found to catalyze certain reactions in its own maturation process. This is contrary to the previously held concept that only proteins could serve as catalysts (enzymes). It also leads to the obvious possibility that RNA molecules were the first self-replicating units of life. This proposal is to investigate the structure of these RNA particles and to determine whether they are present in the most ancient forms of life still present on the planet, the archaeacteria.
RNA的动态能力已经完全实现, 发现RNA分子可以作为催化剂, 中心法则的副作用。 内切核苷酸 RNase P RNA对其底物前体tRNA的活性是 第二个这样的活动将被描述。 提出要 继续研究RNase P的结构, 为了进一步确定催化的机理, RNase P的细胞内结构。此外,提出 扩展对该RNA系统发育学的理解 催化剂以及开始寻找其他生理 重要的小RNA。 RNase P RNA的结构分析 将利用RNase P RNA的比较测序, 几种微生物以及化学和酶探针 在不同温度和离子条件下的结构。 RNase P的遗传学将通过检查 耐辐射异常球菌、玫瑰色微球菌、梭菌 生孢菌、茄嗜热菌和嗜酸热浆菌 使用合成的前体tRNA作为底物。 的 RNase P的细胞内构象将通过 在保护的条件下隔离活动 大分子相互作用,或者,通过问什么, 蛋白质与纯化的RNase P RNA结合。 其他小rna 分离将测试进化保守性(因此, 生理重要性)。 最近发现RNA可以催化某些反应, 自己的成熟过程。 这与此前的 认为只有蛋白质才能作为催化剂 (酶)。 这也导致了一种明显的可能性, 分子是第一个自我复制的生命单位。 这 一项计划是研究这些RNA颗粒的结构 并确定它们是否存在于最古老的 仍然存在于这个星球上的生命形式,古细菌。

项目成果

期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
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Terence Marsh其他文献

Eukaryotic Microbiome of Lake Sturgeon Eggs, and Identification of Chemical Thresholds for Infection Control
  • DOI:
    10.1007/s00248-025-02566-5
  • 发表时间:
    2025-07-24
  • 期刊:
  • 影响因子:
    4.000
  • 作者:
    Kristi Gdanetz;Zachary A. Noel;Ken Saville;Terence Marsh;Kim T. Scribner;Frances Trail
  • 通讯作者:
    Frances Trail
Effects of ammonia fiber expansion (AFEX) treated corn stover on anaerobic microbes and corresponding digestion performance
  • DOI:
    10.1016/j.biombioe.2019.105263
  • 发表时间:
    2019-08-01
  • 期刊:
  • 影响因子:
  • 作者:
    Juan Pablo Rojas-Sossa;Yuan Zhong;Francesca Valenti;John Blackhurst;Terence Marsh;Dana Kirk;Di Fang;Bruce Dale;Wei Liao
  • 通讯作者:
    Wei Liao

Terence Marsh的其他文献

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{{ truncateString('Terence Marsh', 18)}}的其他基金

Novel Oligotrophic Bacteria from the Deep Subsurface Phylogenetically Related to Plastids
来自深层地下的新型寡营养细菌与质体系统发育相关
  • 批准号:
    9707224
  • 财政年份:
    1997
  • 资助金额:
    $ 8.16万
  • 项目类别:
    Continuing Grant
RUI: Structrual Analysis of RNase P, An RNA Catalyst
RUI:RNA 催化剂 RNase P 的结构分析
  • 批准号:
    9096271
  • 财政年份:
    1990
  • 资助金额:
    $ 8.16万
  • 项目类别:
    Standard Grant

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    1990
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