Structure Function Relationships on Cytochrome c

这个项目是在生命过程的化学倡议 （COLP），并由化学和生物物理学联合支持。 蛋白质结构与功能关系的基础研究 将被执行。 利用酵母细胞色素c异构体研究氨基酸 替换和删除会影响关键参数， 电子转移速率，结合强度，特异性 细胞色素C的蛋白质伴侣和蛋白质稳定性。 将进行详细的细胞内测量以进行比较 与体外工作，使用在乳酸培养基中的生长作为 相对比活度的量度。 特定突变具有 为每项研究生成。 沿着 实验工作，各种理论方法， 正在开发，以帮助设计和解释 实验 这个项目是以前的延续 在COLP倡议的支持下。

This project is in the Chemistry of Life Processes Initiative (COLP) and supported jointly by Chemistry and Biophysics. Fundamental studies of protein structure-function relationships will be carried out. Iso-cytochrome c from yeast is used to study how amino acid replacements and deletions affect key parameters such as electron transfer rates, binding strength, specificity to protein partners of cytochrome c, and protein stability. Detailed intracellelar measurements will be made for comparison with the in vitro work, using growth in lactate medium as a measure of relative specific activity. Specific mutations have been generated for each of the studies. Along with the experimental work, a variety of theoretical approaches are being developed to aid in the design and interpretation of experiments. This project is a continuation of that previously supported under the COLP Initiative.