Enzymatic Reactions Involved in Nucleic Acid Metabolism

核酸代谢中涉及的酶反应

• 批准号: 8806960
• 负责人: Jerard Hurwitz
• 金额: $ 33.8万
• 依托单位: Sloan Kettering Institute For Cancer Research
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8806960&HistoricalAwards=false
• 关键词: Enzymatic; Reactions; Involved; Nucleic; Acid

The P.I. plans to study the splicing of pre mRNAs carried out by HeLa nuclear preparations. This project will focus on the separation and charaterization of proteins and the U-rich RNAs that are involved in this process. He will continue to study the interaction of SnRNPs with pre-mRNA as well as auxiliary activities that are directly involved in splicing or contribute to the specificity of this system. He specifically plans to examine the synthesis of SnRNPs from RNA and proteins that constitute these particles. For this purpose, he plans to use labeled RNAs snythesized from cloned DNA sequences that code for the U-rich RNAs. The proteins that interact with these RNAs will also be isolated from HeLa cells. The goal of this approach is to reconstitute SnRNPs and to study their role in the splicing of pre-mRNAs in higher eukaryotes. The P.I. also proposes to examine the replication of bovine papilloma virus with the goal of determining how the replication of this DNA is regulated by the host cell cycle. He has initiated studies on the replication of this DNA by cell-free extracts from mouse cells carrrying a BPV plasmid. Significance: In recent years it has been learned that ribonucleic acid (RNA) as well as proteins can act as catalysts (enzymes) of metabolic reactions. In the former case the reactions catalyzed have been restricted to those splicing out pieces of the RNA transcipts from gene to form a "messenger" RNA of reduced size. The applicant proposes to continue his studies of the mechanism by which these RNA catalyzst are put together.

私家侦探计划研究前mRNA的剪接， 海拉核武器的准备工作 该项目将重点关注 蛋白质和富铀物质的分离和表征 参与这一过程的RNA。 他将继续 研究SnRNP与前mRNA的相互作用， 直接参与剪接的辅助活动， 有助于这个系统的特殊性。 他特别 研究从RNA和蛋白质合成SnRNP的计划 组成这些粒子的物质 为此，他计划 使用从克隆的DNA序列中合成的标记RNA， 富含铀的RNA的编码 蛋白质与 这些RNA也将从HeLa细胞中分离。 的目标 这种方法是重建SnRNP并研究它们的作用 在高等真核生物前体mRNA的剪接中。 私家侦探还建议检查复制 牛乳头状瘤病毒，目的是确定 这种DNA的复制受宿主细胞周期的调节。 他开始研究这种DNA的复制， 来自携带BPV质粒的小鼠细胞的无细胞提取物。 意义：近年来，据了解， 核糖核酸（RNA）和蛋白质一样可以作为催化剂 （酶）代谢反应。 在前一种情况下， 催化的反应仅限于那些 RNA片段从基因中转录出来形成“信使”RNA 缩小尺寸。 申请人拟继续学业 这些RNA催化剂结合在一起的机制