Postdoctoral Research Fellowship in Plant Biology

植物生物学博士后研究奖学金

• 批准号: 8807308
• 负责人: Laura Green
• 金额: $ 5.28万
• 依托单位: Individual Award
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-10-01 至 1990-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8807308&HistoricalAwards=false
• 关键词: Postdoctoral; Research; Fellowship; Plant; Biology

The regulation of carbon metabolism in non-photosynthetic tissues (sinks) of higher plants is poorly understood, despite the fact that these tissues constitute the bulk of the world's supply of food. Photosynthetically derived carbon is generally translocated to sinks as sucrose but, once unloaded, may be processed in different ways. Depending on the type of sink, the imported sucrose may be used predominately in biosynthesis and respiration, or stored, either as sucrose or starch. Little is known about the biochemical mechanisms by which this partitioning is accomplished. These studies will make significant contributions to the understanding of carbon metabolism in sink tissues, an area of plant biology in urgent need of development. Fructose 2,6-bisphosphate (F2,6P2), a regulatory molecule shown to control carbon partitioning in photosynthetic cells, may play a related role in sinks as well. The concentration of F2,6P2 is determined by the activities of fructose 6-phosphate, 2-kinase (F6P, 2K) and fructose 2,6-bisphosphatase (F2,6P2ase), which synthesize and degrade F2,6P2. The Fellow will purify F6P,2K and F2,6P2ase from a variety of sink types to determine whether differences in the properties of the enzymes correlate with different patterns of carbon partitioning. With the purified preparations, the affinity of the enzymes for their substrates and their sensitivity to metabolite effectors will be measured. It will be determined whether sink F6P,2K and F2,6P2ase reside on separate proteins as in leaves, and tested whether the enzymes might be regulated by covalent modification (for example, phosphorylation).

非光合组织碳代谢的调节 （汇）的高等植物知之甚少，尽管事实上， 这些组织构成了世界食物供应的大部分。 光合作用产生的碳通常转移到汇， 蔗糖，但是一旦卸载，可以以不同的方式加工。 根据水槽的类型，可使用进口蔗糖 主要用于生物合成和呼吸，或储存， 蔗糖或淀粉。 人们对生物化学机制知之甚少， 这一划分是完成的。 这些研究将使 对理解碳代谢的重要贡献 库组织是植物生物学中一个亟待发展领域。 果糖2，6-二磷酸（F2，6P 2），一种调节分子， 控制光合作用细胞中的碳分配， 也是水槽的作用。 F2，6P 2的浓度由以下确定： 果糖6-磷酸，2-激酶（F6 P，2K）和果糖 2，6-二磷酸酶（F2，6P 2 ase），其合成并降解F2，6P 2。 研究员将从各种水槽类型中纯化F6 P，2K和F2，6P 2酶 以确定酶的特性差异 与不同的碳分配模式有关。 用纯化的制剂，酶对其的亲和力被降低。 底物及其对代谢物效应物的敏感性 测定了 将确定是否sink F6 P，2K和F2，6P 2ase 存在于单独的蛋白质上，就像在叶子中一样，并测试了酶是否 可以通过共价修饰来调节（例如， 磷酸化）。