Functions of the REPI Region of Plasmid ColV-K30 in Escherichia coli K12

这个项目的目标是识别和描述PColV中存在的功能。k30repi区域与它的复制控制有关。这个复制子是复制的一部分。aerobactin铁。我们发现在携带有氧肌动蛋白铁转运系统基因的质粒中保守的转运单元。REPI复制区赋予细胞与IncFI组相对应的不相容特性，并且在有氧肌动蛋白铁转运系统的保存和流行病学传播中发挥了重要作用。虽然它是细菌质粒中经常发现的复制子，但人们对其复制的控制机制和导致IncFI不相容表型表达的机制知之甚少。正在进行以下实验：1。含有REPI的质粒复制控制中涉及的产物分析。2. 反式的表征。RepI复制所必需的作用蛋白，及其作用机制。3. 阐明新的不相容区incF和已知的incE在导致IncFI表型的REPI不相容机制中的作用。迄今为止，克罗萨博士对这种细菌质粒的研究已经建立了元素复制的总体框架，未来的工作将进一步阐述他所发现的特征。了解这种复制子的功能将促进我们对质粒复制和不同质粒之间兼容性的理解。此外，这项研究可以提高我们对这些质粒如何在细菌群体中传播和维持的理解。

The goal of this project is to identify and characterize the functions present in the PColV.K30 REPI region that are associated with control of its replication. This replicon is part of a replication.aerobactin iron.transport unit that we found to be conserved in plasmid carrying the aerobactin iron-transport system genes. The REPI replication region endows the cells with incompatibility properties corresponding to the IncFI group and must have been instrumental in the preservation and epidemiological spread of the aerobactin iron-transport system. Although it is a replicon frequently found in bacterial plasmid, little is known on the mechanisms controlling its replication and the mechanisms that result in the expression of the IncFI incompatibility phenotype. The following experiments are being performed: 1. Analysis of the product(s) involved in the control of replication of REPI containing plasmid. 2. Characterization of a trans.acting protein RepI which is necessary for REPI replication, and its mechanism of action. 3. Elucidation of the role of the novel incompatibility region incF and the already known incE in the mechanisms of REPI incompatibility that result in the IncFI phenotype. Dr. Crosa's work on this bacterial plasmid to date hasestablished the overall framework of the elements replication, and the proposed future work deals with further elaboration of the features he has found. Understanding of how this replicon functions will certainly advance our understanding of plasmid replication and compatibility between different plasmid. In addition this study may improve our understanding of how these plasmid spread and are maintained in bacterial populations.