Functions of the REPI Region of Plasmid ColV-K30 in Escherichia coli K12

大肠杆菌K12中质粒ColV-K30 REPI区的功能

• 批准号: 8807735
• 负责人: Jorge Crosa
• 金额: $ 37.82万
• 依托单位: Oregon Health & Science University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-15 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8807735&HistoricalAwards=false
• 关键词: Functions; REPI; Region; Plasmid; ColV

The goal of this project is to identify and characterize the functions present in the PColV.K30 REPI region that are associated with control of its replication. This replicon is part of a replication.aerobactin iron.transport unit that we found to be conserved in plasmid carrying the aerobactin iron-transport system genes. The REPI replication region endows the cells with incompatibility properties corresponding to the IncFI group and must have been instrumental in the preservation and epidemiological spread of the aerobactin iron-transport system. Although it is a replicon frequently found in bacterial plasmid, little is known on the mechanisms controlling its replication and the mechanisms that result in the expression of the IncFI incompatibility phenotype. The following experiments are being performed: 1. Analysis of the product(s) involved in the control of replication of REPI containing plasmid. 2. Characterization of a trans.acting protein RepI which is necessary for REPI replication, and its mechanism of action. 3. Elucidation of the role of the novel incompatibility region incF and the already known incE in the mechanisms of REPI incompatibility that result in the IncFI phenotype. Dr. Crosa's work on this bacterial plasmid to date hasestablished the overall framework of the elements replication, and the proposed future work deals with further elaboration of the features he has found. Understanding of how this replicon functions will certainly advance our understanding of plasmid replication and compatibility between different plasmid. In addition this study may improve our understanding of how these plasmid spread and are maintained in bacterial populations.

该项目的目标是识别和表征 存在于PColV.K30 REPI区域中的功能， 与控制其复制有关。 这个复制子 是复制的一部分。有氧肌动蛋白铁。运输单位， 我们发现在携带需氧菌素的质粒中是保守的 铁转运系统基因 REPI复制区域 赋予细胞相应的不相容性 IncFI集团，必须在 需氧菌素的保存和流行病学传播 铁运输系统 虽然它是一种复制子， 在细菌质粒中发现，对细菌质粒的 控制其复制的机制以及 导致IncFI不相容性的表达 表型 正在进行以下实验： 1. 控制中涉及的产品分析 含有REPI的质粒的复制。 2. 一种反式作用蛋白RepI的表征， REPI复制所必需的，及其作用机制。 3. 阐明新的不相容性的作用 区域incF和已知的因斯的机制， 导致IncFI表型的REPI不相容性。 克罗萨博士迄今为止对这种细菌质粒的研究 建立了要素的总体框架 建议今后的工作涉及进一步的 他发现的特征的详细阐述。 这种复制子的功能将有助于我们 了解质粒复制和 不同的质粒 此外，这项研究可能会提高我们的 了解这些质粒如何传播和维持 在细菌种群中。