Expedited Award for Aqueous 2-Phase Extraction

研究的目的是发展一种新的方法， 在水溶液中使用的昂贵的亲和配体的损失 用于生物分离的两相分配方法， 生物净化 具体研究的是一种新型的生物活性物质的纯化方法。 蛋白质在由葡聚糖、 高分子量聚乙二醇（PEG） 配体，和牺牲的低分子量PEG，其更 与葡聚糖相容，因此它最大限度地减少了连接的 将PEG加入葡聚糖相中。 配体的损失是商业化的主要障碍。 用于生物分离的含水两相分配方法， 生物净化 这项研究的成功可能会加速 分区过程的商业化。

The aim of the research is to develop a new method for minimizing the loss of the expensive affinity ligands used in aqueous two-phase partition processes for bioseparation and biopurification. The specific study is on the purification of a protein in an aqueous two-phase system consisting of dextran, a high molecular-weight polyethylene glycol (PEG) with attached ligand, and a sacrificial low molecular-weight PEG which is more compatible with dextran so it minimizes the loss of the ligated PEG into the dextran phase. Loss of ligands is a major impediment to the commercialization of the aqueous two-phase partitioning process for bioseparation and biopurification. Success of the research could accelerate the commercialization of the partition process.