Expedited Award for Aqueous 2-Phase Extraction

水相两相萃取加急奖

• 批准号: 8812343
• 负责人: Stanley Sandler
• 金额: $ 3万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8812343&HistoricalAwards=false
• 关键词: Expedited; Award; Aqueous; Phase; Extraction

The aim of the research is to develop a new method for minimizing the loss of the expensive affinity ligands used in aqueous two-phase partition processes for bioseparation and biopurification. The specific study is on the purification of a protein in an aqueous two-phase system consisting of dextran, a high molecular-weight polyethylene glycol (PEG) with attached ligand, and a sacrificial low molecular-weight PEG which is more compatible with dextran so it minimizes the loss of the ligated PEG into the dextran phase. Loss of ligands is a major impediment to the commercialization of the aqueous two-phase partitioning process for bioseparation and biopurification. Success of the research could accelerate the commercialization of the partition process.

这项研究的目的是开发一种新的方法，以最大限度地减少用于生物分离和生物纯化的双水相分配过程中昂贵的亲和配体的损失。具体的研究是在双水相体系中纯化蛋白质，该双水相体系由葡聚糖、带有配基的高分子聚乙二醇组分和与葡聚糖更相容的牺牲性低分子聚乙二醇组分组成，从而最大限度地减少了连接的聚乙二醇组分进入葡聚糖相的损失。配体的损失是阻碍用于生物分离和生物纯化的双水相分配过程商业化的主要障碍。研究的成功可能会加速分割进程的商业化进程。