Expedited Award for Aqueous 2-Phase Extraction

该研究的目的是开发一种新的方法，以最大限度地减少用于生物分离和生物纯化的水两相分配过程中昂贵的亲和配体的损失。具体的研究是在水两相体系中纯化蛋白质，该体系由葡聚糖、高分子量聚乙二醇（PEG）和附着的配体、以及牺牲的低分子量PEG，其与葡聚糖更相容，从而使连接的PEG在葡聚糖相中的损失最小化。配体的损失是生物分离和生物净化的水两相分配工艺商业化的主要障碍。该研究的成功将加速该分区的商业化进程。

The aim of the research is to develop a new method for minimizing the loss of the expensive affinity ligands used in aqueous two-phase partition processes for bioseparation and biopurification. The specific study is on the purification of a protein in an aqueous two-phase system consisting of dextran, a high molecular-weight polyethylene glycol (PEG) with attached ligand, and a sacrificial low molecular-weight PEG which is more compatible with dextran so it minimizes the loss of the ligated PEG into the dextran phase. Loss of ligands is a major impediment to the commercialization of the aqueous two-phase partitioning process for bioseparation and biopurification. Success of the research could accelerate the commercialization of the partition process.