US-United Kingdom Cooperative Research: Assembly of the Neuronal Cytoskeleton

该奖项将支持詹姆斯博士之间的合作研究 班堡，科罗拉多州立大学，和丹尼斯·布雷博士，细胞 生物物理部，医学研究所，伦敦。 在这个项目中 Bamburg博士和Bray博士将研究 肌动蛋白进入神经元皮层使用荧光和电子 显微镜 肌动蛋白丝是主要的细胞骨架元素， 神经元生长锥，并包括核心结构， 微刺，伪足从生长锥延伸， 其产生定向神经突生长所需的张力。 Bamburg博士和Bray博士计划确定 肌动蛋白和其他蛋白质运输到轴突皮层。 的 拟议的实验应该确定肌动蛋白亚基是否交换 在运输过程中轴突中的细丝群之间， 如果轴突的皮质肌动蛋白层是由沉积在 生长锥或轴。 在这个项目的过程中，班伯格博士将准备 用于显微注射的蛋白质;布雷博士将制备抗体 与荧光标记进行对比。 显微注射研究将 在两个实验室进行。 拟议的研究是 非常适合这两个实验室之间的合作。 博士 布雷一直是神经元培养发展的领导者 方法，产生神经元细胞蛋白的抗体，和 免疫细胞化学方法在其他 蛋白质在神经元内的定位。 班伯格医生 在蛋白质的体外分离和表征中具有活性 ________ 参与细胞骨架结构和调节 细胞骨架成分

This award will support collaborative research between Dr. James Bamburg, Colorado State University, and Dr. Dennis Bray, Cell Biophysics Unit, Medical Research Coucil, London. In this project Dr. Bamburg and Dr. Bray will investigate the sites of assembly of actin into the neuronal cortex using fluorescence and electron microscopy. Actin filaments are the major cytoskeletal element of the neuronal growth cone and comprise the core structure of microspikes, pseudopodia which extend from the growth cone and which generate the tension required for directed neurite growth. Drs. Bamburg and Bray plan to identify putative complexes between actin and other proteins in transit to the axonal cortex. The proposed experiments should determine if actin subunits exchange between the filament population in the axon during transport, and if the cortical actin layer of the axon arises from deposition at the growth cone or the shaft. During the course of the project, Dr. Bamburg will prepare proteins for microinjection; Dr. Bray will prepare antibodies against the fluorescent labels. The microinjections studies will be carried out in both laboratories. The proposed studies are well suited for collaboration between these two laboratories. Dr. Bray has been a leader in the development of neuronal culture methods, production of antibodies to neuronal cell proteins, and the application of immunocytochemical methods for other localization of proteins within neurons. Dr. Bamburg has been active in isolation and characterization in vitro of proteins ________ involved in cytoskeletal structures and in regulating assembly of cytoskeletal components.