Cloning of the cDNA Encoding Plant Calcium-Dependent Protein Kinase

编码植物钙依赖性蛋白激酶 cDNA 的克隆

• 批准号: 8816992
• 负责人: Alice Harmon
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-10-15 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8816992&HistoricalAwards=false
• 关键词: Cloning; cDNA; Encoding; Plant; Calcium

Soybean calcium-dependent protein kinase (CDPK) is the first Ca2+-dependent protein kinase from plants to be purified to homogeneity. This enzyme represents a new class of protein kinases that are dependent on micromolar calcium for activity but do not require phospholipids or calmodulin for activity. Preliminary immunocytochemical localization of the enzyme shows that CDPK is associated with actin filaments in soybean and onion root tip cells. This association suggests that CDPK may play a role in the regulation of plant actin. The purification and characterization of CDPK allows the study of both the role of calcium and protein phosphorylation in plants. This research investigates these roles by (1) fully characterizing the calcium-binding properties of CDPK, (2) determining the immunological properties of CDPK, (3) determining how CDPK binds to actin, and (4) identifying actin-associated proteins that are substrates of CDPK.%%% Many metabolic activities in plants and animals are regulated by phosphorylation/dephosphorylation mechanisms. Protein kinases play a major role in this type of regulation. Recently, a new class of protein kinases which directly bind calcium have been discovered. The experiments defined here will provide for investigation of the potential role of these calcium-dependent protein kinases in the regulation of the plant cytoskeleton and to better define its role in the regulation of plant cell function and in plant growth and development.***//

大豆钙依赖蛋白激酶（CDPK）是第一个 来自植物的Ca 2+依赖性蛋白激酶， 同质性 这种酶代表了一类新的蛋白质 依赖微摩尔钙活性的激酶 但活性不需要磷脂或钙调蛋白。 酶的初步免疫细胞化学定位 表明CDPK与大豆中的肌动蛋白丝相关， 和洋葱根尖细胞。 这种关联表明， CDPK可能在植物肌动蛋白的调节中发挥作用。 的 CDPK的纯化和表征允许研究 钙和蛋白质磷酸化在 植物 本研究通过（1）充分 表征CDPK的钙结合性质，（2） 确定CDPK的免疫学特性，（3） 确定CDPK如何与肌动蛋白结合，以及（4）鉴定 作为CDPK底物的肌动蛋白相关蛋白。% 植物和动物的许多代谢活动都受到调节 通过磷酸化/去磷酸化机制。 蛋白 激酶在这类调节中起主要作用。 最近，一类新的蛋白激酶， 钙被发现了。 这里定义的实验 将提供调查这些潜在的作用， 钙依赖性蛋白激酶在调节 植物细胞骨架并更好地定义其在植物中的作用 调节植物细胞功能和植物生长， 发展。*/