Regulation of the Metabolic Processes of the single cell at the Molecular level

分子水平上单细胞代谢过程的调控

• 批准号: 8817091
• 负责人: Boris Magasanik
• 金额: --
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8817091&HistoricalAwards=false
• 关键词: Regulation; Metabolic; Processes; single; cell

The object of the proposed research is to elucidate the mechanism responsible for the activation of transcription at the nitrogen-regulated promoter glnAp2 of the structural gene for glutamine synthetase in Escherichia coli. We have shown that core RNA polymerase with sigma54, the specific o factor for nitrogen regulated promoters, combines with glnAp2 in a closed complex. The activator NRI, after phosphorylation by the modulator NRII stimulates the isomerization to the open complex. We plan to separate the open complexes from other proteins and small molecules and to probe the interaction of NRI- phosphate with the DNA of the promoter and with sigma54. We shall also attempt to discover the mechanism responsible for the ability of NRI to activate in intact cells the transcription of glnAp2 in the absence of NRII by the isolation and study of mutants altered in this ability. We shall attempt to identify the DNA sites in the vicinity of glnAp2 that are responsible for the dampening of glnAp2 transcription by an increased intracellular concentration of NRI.

拟议研究的目的是阐明 负责转录激活的机制， 结构基因的氮调节启动子glnAp 2， 用于大肠杆菌中谷氨酰胺合成酶。 我们已经表明 核心RNA聚合酶与sigma 54，特异性的o因子， 对于氮调节启动子，与glnAp 2结合， 封闭的复合体 激活剂NRI，在磷酸化后， 调节剂NRII刺激异构化开放 复杂. 我们计划将开放式建筑群与其他建筑群分开 蛋白质和小分子的相互作用， NRI-磷酸与启动子的DNA和σ 54。 我们还将试图发现负责的机制 NRI在完整细胞中激活的能力， 在不存在NRII的情况下，通过分离GlnAp 2， 并研究这种能力的突变体。 我们将试图 为了鉴定glnAp 2附近的DNA位点， 负责抑制glnAp 2的转录， 增加细胞内NRI浓度。