ROW/RIA: Interrelationships of Salamanders of the genus Ambystoma

ROW/RIA：钝口蝾螈属的相互关系

• 批准号: 8818630
• 负责人: Christina Spolsky
• 金额: $ 21.26万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8818630&HistoricalAwards=false
• 关键词: ROW; RIA; Interrelationships; Salamanders; genus

The proposed study will provide the first comprehensive analysis of evolutionary relationships in the genus Ambystoma, the mole salamanders. Relationships will be determined by measuring genetic differences in mitochondrial DNA. With a well supported phylogenetic tree, one can study the biogeographical factors involved in the adaptive radiation of the group. This part of the study will also provide information on the mechanisms of evolution of mitochondrial DNA in amphibians, a group for which to be used in this study may prove useful for determining genetic relationships in many other biological systems. A good phylogeny will provide the context for the second part of this study. The mole salamander genus includes several distinctive clonally reproducing (unisexual) forms; these originated by hybridization between different species of Ambystoma. In the second part of the proposed study, mitochondrial DNA will be used to analyze genetic variation in the clonal populations. This will provide information on the maternal ancestors of clonal forms. It will also show whether there were many or few such origins, recent or ancient. One will therefore be able to reconstruct a history of these lineages-- where did they come from, how, when, and to what extent did they spread. Such an evolutionary history will provide the necessary background for more detailed studies of the genetic mechanisms involved in producing clonal organisms.

这项研究将首次对鼹鼠蝾螈属的进化关系进行全面分析。 关系将通过测量线粒体 DNA 的遗传差异来确定。 有了良好支持的系统发育树，人们就可以研究与群体适应性辐射有关的生物地理因素。 这部分研究还将提供有关两栖动物线粒体 DNA 进化机制的信息，本研究中使用的一个群体可能有助于确定许多其他生物系统中的遗传关系。 良好的系统发育将为本研究的第二部分提供背景。 鼹鼠蝾螈属包括几种独特的克隆繁殖（单性）形式；这些起源于不同种类的钝口螈之间的杂交。 在拟议研究的第二部分中，线粒体 DNA 将用于分析克隆群体的遗传变异。 这将提供有关克隆形式的母系祖先的信息。 它还将显示这样的起源是多还是少，是近代的还是远古的。 因此，人们将能够重建这些谱系的历史——它们来自哪里、如何、何时以及传播到什么程度。 这样的进化史将为更详细地研究克隆生物产生的遗传机制提供必要的背景。