Molecular Mapping of the Chlamydomonas Genome

单细胞绿藻莱茵衣藻（Chlamydomonas reinhardtii）具有几个独特的特征，使其成为真核鞭毛、叶绿体、细胞-细胞相互作用和许多生化途径相关基因的分子遗传学研究的模型系统。我们将开发衣藻基因组的分子图谱，以补充目前存在的广泛的遗传图谱。限制性内切片段长度多态性将用于区分两个干涉性菌株的DNA，以跟踪遗传杂交中分子标记的分离。分子标记将与19个连锁组的表型标记相对应。在每一个连锁组上，将有一系列的连锁分子标记。利用与端粒相关的分子标记来定位连锁基团的末端。通过在基因组的一个区域进行“染色体行走”，将寻求分子图谱和遗传图谱之间的精确相关性。该项目将极大地促进包括我们自己的实验室在内的许多实验室正在进行的衣藻核基因的分子和遗传分析。单细胞绿藻衣藻已成为研究许多基础生物学问题的重要模式生物。这是一项发展详细和全面的DNA和这种生物的基因图谱的建议。这样的地图将是该领域工作人员的宝贵资源，并将大大便利他们的工作。

The unicellular green alga Chlamydomonas reinhardtii has several unique characteristics that make it the model system of choice for molecular genetic studies of genes involved in eukaryotic flagella, chloroplasts, cell-cell interactions, and a number of biochemical pathways. We will develop a molecular map of the Chlamydomonas genome to complement the extensive genetic map that currently exists. Restriction fragment length polymorphism that distinguish the DNA from two interfertile strains will be used to follow the segregation of molecular markers in genetic crosses. Molecular markers will be mapped with respect to phenotypic markers on each of the nineteen linkage groups. Sets of linked molecular markers will be ordered on each linkage group. The ends of linkage groups will be mapped using molecular markers associated with telomere. Precise correlation between the molecular map and the genetic map will be sought by carrying out a "chromosome walk" in one region of the genome. This project will greatly facilitate molecular and genetic analysis of Chlamydomonas nuclear genes currently underway in many laboratories including our own. The single-celled green alga Chlamydomonas has become an important model organism for the study of a number of problems of fundamental biological interest. This is a proposal to develop a detailed and comprehensive map of the DNA and genes of this organism. Such a map will be a valuable resource to workers in the field, and will greatly facilitate their work.