Analysis of VirB Proteins of A. Tumefaciens Required for T-DNA Transfer

T-DNA 转移所需的根瘤土壤杆菌 VirB 蛋白分析

• 批准号: 8819197
• 负责人: Andrew Binns
• 金额: --
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-15 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8819197&HistoricalAwards=false
• 关键词: Analysis; VirB; Proteins; A.; Tumefaciens

Ward Virulent strains of Agrobacterium tumefaciens initiate tumors on plants by transferring the T-DNA from its resident Ti plasmid into plant cells. A productive interaction between bacterium and plant cell requires the appropriate attachment of the bacterium to the plant cell, and expression of a series of virulence (vir) genes in the bacterium. While the functions of several vir proteins are known, the mechanism by which the T-DNA is transported across two sets of cell walls and membranes is not known. Recent data supports the hypothesis that the mechanism of DNA transfer is analogous to that of bacterial conjugation, suggesting that a DNA transfer apparatus, such as the conjugal pore encoded by the F plasmid of E. coli, is involved. The virB operon is the logical candidate to encode the proteins of such a pore. The possible role of virB proteins in the transfer of T- DNA from the bacterium to the plant cell will be investigated using in vitro mutagenesis and recombinant DNA techniques, immunocharacterization and immunolocalization of the proteins, and biochemical approaches to determine whether or not the proteins are involved in physical interactions with bacterial membranes or DNA. Currently, plant biotechnology is heavily dependent on gene transfer based on Agrobacterium. Some plants are readily transformable by this vector, while others are not. Thus, elucidating the mechanism of DNA transfer in this system will be of great importance to plant genetic engineering.

病房 根癌农杆菌的毒力菌株引发肿瘤 通过将T-DNA从其驻留Ti质粒转移到植物上 转化成植物细胞。 细菌和 植物细胞需要细菌的适当附着 并表达一系列毒力（vir） 细菌中的基因。 虽然几个病毒的功能 蛋白质是已知的，T-DNA的机制是 通过两组细胞壁和细胞膜运输的蛋白质 知道的 最近的数据支持这一假设，即 DNA转移类似于细菌接合， 这表明DNA转移装置，如配偶体， E.大肠杆菌，参与其中。 VirB 操纵子是编码这种蛋白质的逻辑候选者。 毛孔 virB蛋白在T-淋巴细胞转移中的可能作用 将研究从细菌到植物细胞的DNA 使用体外诱变和重组DNA技术， 蛋白质的免疫表征和免疫定位， 和生物化学方法来确定 蛋白质参与了与细菌的物理相互作用 膜或DNA。 目前，植物生物技术严重依赖于基因 基于农杆菌的转移。 有些植物很容易 可以被这个载体转化而其他的则不能 因此，在本发明中， 阐明该系统中DNA转移的机制将是 这对植物基因工程非常重要。