Transcription of Class II Genes: Mechanisms of Activation

该实验室的一个长期目标是能够通过对所涉及的蛋白质及其各种蛋白质：DNA和蛋白质：蛋白质的相互作用的表征，详细描述在蛋白质编码基因II类基因的转录启动过程中发生的事件，目的是制定和了解一些作用于II类基因表达调控的机制。其具体目的是了解转化基因(如ElA)调节其他病毒和细胞基因表达的机制(S)。这些研究包括分离转录受Ela基因产物调控的基因所需的因子。这些因子将通过功能转录实验从腺病毒感染和未感染的细胞中纯化出来。基因特异性因子与Ela蛋白、其他转录因子和启动子序列的相互作用将通过技术进行分析。了解转录调控的机制是极其重要的。许多研究报道了基于DNA结合分析获得可能的转录因子。虽然这种方法已经产生了重要的信息，但是否继续取得进展将取决于对体外转录功能的详细生化分析系统。由于真核生物polIII转录的复杂性以及参与调控的无数蛋白质因子必须是大量提纯或部分提纯的成分。莱因伯格博士是致力于这项任务的为数不多的研究人员之一。

A long range goal of this laboratory is to be able to detail the events that occur during the initiation of transcription of protein coding genes class II genes through the characterization of the proteins involved and their various protein: DNA and protein:protein interactions with the intent of formulating and understanding some of the mechanisms that operate in the regulation of expression of class II genes. The specific aims are to understand the mechanism(s) by which a transforming gene such as Ela can regulate the expression of other viral and cellular genes. These include studies on the isolation of the factors required for transcription of genes whose expression is regulated by the products of the Ela gene. The factors will be purified from adenovirus infected and uninfected cells using functional transcription assay. The interaction of the gene specific factor with Ela protein, other transcription factors and promoter sequences will be analyzed through the use of techniques. Understanding the mechanisms by which transcription is regulated is extremely important. A number of studies report obtaining putative transcription factors on the basis of DNA binding assays. While this approach has yielded important information continued progress is going to depend on detailed biochemical analysis of functional in vitro transcription systems. Because of the complexity of eucaryotic pol III transcription and the myriad of protein factors involved in modulation there must be a large number of purified or partially purified components. Dr. Reinberg is one of the few investigators dedicated to this task.