Transcription of Class II Genes: Mechanisms of Activation

这个实验室的一个长期目标是能够详细 在转录起始期间发生的事件 蛋白质编码基因II类基因通过 所涉及的蛋白质及其各种 蛋白质：DNA和蛋白质：蛋白质相互作用， 形成和理解一些机制， 在调节II类基因的表达中起作用。 的 具体目标是了解一个 转化基因如Ela可以调控 其他病毒和细胞基因。 这些研究包括： 基因转录所需因子的分离 其表达受Ela基因产物调控。 这些因子将从腺病毒感染的腺病毒中纯化， 未感染的细胞，使用功能性转录测定。 的 基因特异性因子与Ela蛋白的相互作用，其他 转录因子和启动子序列将被分析 通过使用技术。 理解转录的机制 监管是非常重要的。 一些研究报告指出， 基于DNA获得推定的转录因子， 结合测定。 虽然这种方法产生了重要的 信息持续进展将取决于详细信息 功能性体外转录的生化分析 系统. 由于真核聚合酶III的复杂性， 转录和无数的蛋白质因子参与 调制必须有大量的纯化或 部分纯化的组分。 Reinberg医生是为数不多的 调查人员致力于这项任务。

A long range goal of this laboratory is to be able to detail the events that occur during the initiation of transcription of protein coding genes class II genes through the characterization of the proteins involved and their various protein: DNA and protein:protein interactions with the intent of formulating and understanding some of the mechanisms that operate in the regulation of expression of class II genes. The specific aims are to understand the mechanism(s) by which a transforming gene such as Ela can regulate the expression of other viral and cellular genes. These include studies on the isolation of the factors required for transcription of genes whose expression is regulated by the products of the Ela gene. The factors will be purified from adenovirus infected and uninfected cells using functional transcription assay. The interaction of the gene specific factor with Ela protein, other transcription factors and promoter sequences will be analyzed through the use of techniques. Understanding the mechanisms by which transcription is regulated is extremely important. A number of studies report obtaining putative transcription factors on the basis of DNA binding assays. While this approach has yielded important information continued progress is going to depend on detailed biochemical analysis of functional in vitro transcription systems. Because of the complexity of eucaryotic pol III transcription and the myriad of protein factors involved in modulation there must be a large number of purified or partially purified components. Dr. Reinberg is one of the few investigators dedicated to this task.