Transcription of Class II Genes: Mechanisms of Activation

II 类基因的转录：激活机制

• 批准号: 8819342
• 负责人: Danny Reinberg
• 金额: $ 29.8万
• 依托单位: Rutgers, The State University of New Jersey-RBHS-Robert Wood
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8819342&HistoricalAwards=false
• 关键词: Transcription; Class; II; Genes; Mechanisms

A long range goal of this laboratory is to be able to detail the events that occur during the initiation of transcription of protein coding genes class II genes through the characterization of the proteins involved and their various protein: DNA and protein:protein interactions with the intent of formulating and understanding some of the mechanisms that operate in the regulation of expression of class II genes. The specific aims are to understand the mechanism(s) by which a transforming gene such as Ela can regulate the expression of other viral and cellular genes. These include studies on the isolation of the factors required for transcription of genes whose expression is regulated by the products of the Ela gene. The factors will be purified from adenovirus infected and uninfected cells using functional transcription assay. The interaction of the gene specific factor with Ela protein, other transcription factors and promoter sequences will be analyzed through the use of techniques. Understanding the mechanisms by which transcription is regulated is extremely important. A number of studies report obtaining putative transcription factors on the basis of DNA binding assays. While this approach has yielded important information continued progress is going to depend on detailed biochemical analysis of functional in vitro transcription systems. Because of the complexity of eucaryotic pol III transcription and the myriad of protein factors involved in modulation there must be a large number of purified or partially purified components. Dr. Reinberg is one of the few investigators dedicated to this task.

该实验室的长期目标是能够通过所涉及的蛋白质及其各种蛋白质：DNA和蛋白质：蛋白质相互作用的表征，详细描述蛋白质编码基因II类基因转录起始过程中发生的事件，目的是制定和理解一些在II类基因表达调节中起作用的机制。 具体目标是了解 Ela 等转化基因调节其他病毒和细胞基因表达的机制。 其中包括分离基因转录所需因子的研究，这些基因的表达受Ela基因产物的调节。 使用功能转录测定从腺病毒感染和未感染的细胞中纯化这些因子。 通过技术分析基因特异性因子与Ela蛋白、其他转录因子和启动子序列的相互作用。 了解转录调控机制极其重要。 许多研究报告在 DNA 结合测定的基础上获得了假定的转录因子。 虽然这种方法已经产生了重要的信息，但持续的进展将取决于功能性体外转录系统的详细生化分析。 由于真核 pol III 转录的复杂性和参与调节的无数蛋白质因子，必须有大量纯化或部分纯化的成分。 赖因伯格博士是少数致力于这项任务的研究人员之一。