Acquisition of an Amino Acid Analyzer

该提案要求提供资金，用于购买国家- 具有高度灵敏度的氨基酸分析仪， 夏威夷大学生物技术-分子生物学 仪器设施。 该设施已被组装， 所有需要服务的大学教师的共享使用， 蛋白质测序、肽和寡核苷酸合成， 在“收费服务”的基础上提供相关服务。 但 该设施目前缺乏现代化的氨基酸分析仪， 教师对蛋白质组成数据的需求没有得到满足。 校园里唯一的氨基酸分析仪， 专门用于一个实验室，是贝克曼121 MB，是11岁。 它在敏感性方面已经过时， 由于缺少备件而难以维修。 具有这种 在大陆进行常规分析是不现实的， 夏威夷的地理隔离。 缺乏氨基酸 分析仪是一个主要的缺陷，如果得到纠正， 大大提高核心设施的能力， 提供大分子表征服务， 大学研究社区。

This proposal requests funding for the purchase of a state-of- the-art amino acid analyzer with a high degree of sensitivity for University of Hawaii's Biotechnology-Molecular Biology Instrumentation Facility. This facility has been assembled for the shared use of all University faculty who require services in protein sequencing, peptide and oligonucleotide synthesis and related services on a "fee-for-service" basis. However, the facility currently lacks a modern amino acid analyzer and the faculty's needs for protein compositional data have gone unmet. The only existing amino acid analyzer on campus, which is dedicated to one lab, is a Beckman 121 MB and is 11 years old. It is outdated in terms of sensitivity and is increasingly difficult to maintain for lack of spare parts. Having such routine analysis done on the mainland is unrealistic because of Hawaii's geographical isolation. The lack of an amino acid analyzer is a major deficiency which if corrected will significantly enhance the capacity of the core facility to provide services in macromolecular characterization for the University research community.