Acquisition of a Transmission Electron Microscope and Accessory Equipment for Image Processing and Analysis

购置透射电子显微镜和用于图像处理和分析的附属设备

• 批准号: 8820805
• 负责人: Venita Allison
• 金额: $ 5.53万
• 依托单位: Southern Methodist University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820805&HistoricalAwards=false
• 关键词: Acquisition; Transmission; Electron; Microscope; Accessory

The present proposal seeks support to obtain a rebuilt Philips EM300 transmission electron microscope. Several major proposed projects require electron microscopy, but are not adequately supported by existing equipment. These research projects utilize combinations of morphological, morphometric and immunolocalization analyses in conjunction with biochemical or molecular techniques. (1) To study the influence of calcium ions on differentiation in Physarum polycephalum. Exogenous calcium can induce differentiation and differentiation is accompanied by mobilization of internal calcium stores. Morphometric analyses are proposed to quantitate changes in the mitochondrial content of stored calcium granules. (2) To study the genetic mechanisms regulating eggshell formation during oogenesis in Drosophila by visualizing the effects of well defined mutations on eggshell development and to localizing with high resolution the responsible gene products. (3) To study molecular mechanisms involved in regulation of the mitotic process to localizing with high resolution monoclonal antibodies MPM-1 and MPM-2 which recognize a subset of phosphorylated proteins abundant in mitotic cells. (4) To study calcium regulatory pathways in Trypanosoma brucei by immunolocalization. (5) To use morphometric analyses to provide ultrastructural evidence of free radical damage during aging in tissues of the housefly, Musca domestica. (6) To evaluate the basis of immunosuppression in mice infected with Trichonella pseudospiralis by analysis of adrenal mitochondria and lipid storage droplets in the zona fasciculate layer to provide further evidence of a parasite induced hyperadrenal state. (7) To evaluate, morphologically, the effect of mutations on ATPase organization. (8) To identify changes in seminal vesicle composition and structure which accompany the aging process. Emphasis is place upon the distribution and quantity of type IV collagen, laminin, proteoglycans and testosterone.

目前的建议寻求支持，以获得重建飞利浦 EM 300透射电子显微镜 几个主要的拟议项目需要电子显微镜，但 现有设备无法充分支持。 这些 研究项目利用形态学， 形态测定和免疫定位分析， 生物化学或分子技术。 (1)为了研究 钙离子对多头绒泡菌分化的影响。 外源钙可诱导分化和分化 伴随着内部钙储存的动员。 形态定量分析提出了定量的变化， 线粒体内钙颗粒的含量。 (2)研究 调控蛋壳形成的遗传机制 通过观察井的影响， 在蛋壳发育上定义的突变， 高分辨率的基因产物。 (3)研究 参与有丝分裂调节的分子机制 用高分辨率单克隆抗体定位的方法 MPM-1和MPM-2识别磷酸化的 有丝分裂细胞中丰富的蛋白质。 (4)研究钙 布氏锥虫的免疫定位调控途径。 (5)利用形态学分析提供超微结构 衰老过程中自由基损伤的证据 家蝇Musca acutica。 (6)评估的基础 毛耳菌感染小鼠免疫抑制 肾上腺线粒体和脂质分析 储存液滴在纤维束层中以提供进一步的 寄生虫导致肾上腺功能亢进的证据 (7)到 从形态学上评估突变对ATP酶的影响， organization. (8)确定精囊的变化 组成和结构，伴随着老化过程。 重点放在第四类的分布和数量上 胶原蛋白、层粘连蛋白、蛋白聚糖和睾酮。