US-Belgium Cooperative Research: Regulation of Phage Mu Transposition

美国-比利时合作研究：噬菌体Mu转座的调控

• 批准号: 8821706
• 负责人: Norman Higgins
• 金额: $ 1.05万
• 依托单位: University of Alabama at Birmingham
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1993-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8821706&HistoricalAwards=false
• 关键词: US; Belgium; Cooperative; Research; Regulation

This award will support collaborative research between a group of U.S. and Belgian biochemists. The U.S. investigators are Dr. N. P. Higgins, University of Alabama at Birmingham and Dr. Martin Pato, University of Colorado Health Science Center. The Belgian researcher is Dr. Ariane Toussaint, Free University of Brussels. The long range goal of this project is to understand regulation of genetic transposition in bacterial populations. The Mu transposase and semi-essential genes participate in a highly efficient replication-transposition reaction in vivo. In this __ ____ project, experiments will be performed to analyze the transposase and early genes that influence Mu biology at the stages of the transcription and DNA synthesis. Objectives of the collaborative work include i) expression, ii) purification and iii) characterization of DNA binding proteins encoded by the virus. The investigators will extend joint efforts to chromosome-associated proteins (HU and other "histone-like" proteins) of E. coli and _ ____ Salmonella typhimurium. This approach will expand basic knowledge __________ ___________ of the chromosomal proteins that organize a functional folded nucleoid. The three investigators run laboratories that have demonstrated complementary expertise in all of the areas to be studied. Systems involving purified proteins for analyzing Mu transcription and replication in vitro are established in __ _____ Birmingham. Methods for analyzing Mu transcription and replication in vivo are established in Denver. The group in Brussels has genetic strategies for selecting mutants and carrying out the genetic dissection of proteins related to Mu biology. By coordinating experiments at the three sites, the investigators can test a hypothesis genetically and measure predicted behavior in ___ vitro and in vivo in a relatively short period of _____ _______ time.

该奖项将支持一组美国和比利时生物化学家之间的合作研究。美国调查人员是阿拉巴马大学伯明翰分校的N·P·希金斯博士和科罗拉多大学健康科学中心的马丁·帕托博士。比利时研究人员是布鲁塞尔自由大学的Ariane Toussaint博士。这个项目的长期目标是了解细菌种群中基因转位的调节。Mu转座酶和半必需基因在体内参与了一个高效的复制-转座反应。在这个_项目中，将进行实验，以分析在转录和DNA合成阶段影响Mu生物学的转座酶和早期基因。合作工作的目标包括i)表达、ii)纯化和iii)鉴定由病毒编码的DNA结合蛋白。研究人员将把联合努力扩展到大肠杆菌和_鼠伤寒沙门氏菌的染色体相关蛋白(HU和其他类似组蛋白的蛋白)。这种方法将扩展关于染色体蛋白质的基本知识_。这三名研究人员管理的实验室在所有要研究的领域都表现出了互补的专业知识。在伯明翰建立了用于体外分析Mu转录和复制的纯化蛋白系统。丹佛建立了体内分析Mu转录和复制的方法。布鲁塞尔的这个小组有选择突变体和进行与Mu生物学相关的蛋白质的遗传解剖的遗传策略。通过协调三个地点的实验，研究人员可以在相对较短的_时间到了。