US-Belgium Cooperative Research: Regulation of Phage Mu Transposition

该奖项将支持一组合作研究， 美国和比利时生物化学家。 美国调查人员是N. P. 希金斯，亚拉巴马大学伯明翰分校和马丁帕托博士， 科罗拉多大学健康科学中心。 比利时 研究人员是布鲁塞尔自由大学的Ariane Toussaint博士。 本项目的长期目标是了解 细菌种群的基因转座。 Mu转座酶和半必需基因参与了一个高度的 体内高效的复制转座反应。 在这 __ ____ 项目，实验将进行分析转座酶 以及影响Mu生物学的早期基因， 转录和DNA合成。 合作的目标 工作包括i）表达，ii）纯化和iii） 病毒编码的DNA结合蛋白的表征。 的 研究人员将共同努力， 蛋白质（HU和其他“组蛋白样”蛋白）。杆菌和 _ ____ 鼠伤寒沙门氏菌。 这种方法将扩大基础知识 __________ ___________ 这些染色体蛋白质组织了一个功能性的折叠的 类核 这三名研究人员经营的实验室 在所有领域展示了互补的专业知识， 研究了 用于分析Mu的包含纯化蛋白质的系统 体外转录和复制建立在 __ _____ 伯明翰 分析Mu转录和复制的方法 在丹佛建立了体内实验室。 在布鲁塞尔的小组已经 选择突变体的遗传策略， 与Mu生物学相关的蛋白质的遗传解剖。 通过 通过协调三个地点的实验，研究人员可以 从遗传学角度检验假设， ___ 在相对较短的时间内， _____ _______ 时间

This award will support collaborative research between a group of U.S. and Belgian biochemists. The U.S. investigators are Dr. N. P. Higgins, University of Alabama at Birmingham and Dr. Martin Pato, University of Colorado Health Science Center. The Belgian researcher is Dr. Ariane Toussaint, Free University of Brussels. The long range goal of this project is to understand regulation of genetic transposition in bacterial populations. The Mu transposase and semi-essential genes participate in a highly efficient replication-transposition reaction in vivo. In this __ ____ project, experiments will be performed to analyze the transposase and early genes that influence Mu biology at the stages of the transcription and DNA synthesis. Objectives of the collaborative work include i) expression, ii) purification and iii) characterization of DNA binding proteins encoded by the virus. The investigators will extend joint efforts to chromosome-associated proteins (HU and other "histone-like" proteins) of E. coli and _ ____ Salmonella typhimurium. This approach will expand basic knowledge __________ ___________ of the chromosomal proteins that organize a functional folded nucleoid. The three investigators run laboratories that have demonstrated complementary expertise in all of the areas to be studied. Systems involving purified proteins for analyzing Mu transcription and replication in vitro are established in __ _____ Birmingham. Methods for analyzing Mu transcription and replication in vivo are established in Denver. The group in Brussels has genetic strategies for selecting mutants and carrying out the genetic dissection of proteins related to Mu biology. By coordinating experiments at the three sites, the investigators can test a hypothesis genetically and measure predicted behavior in ___ vitro and in vivo in a relatively short period of _____ _______ time.