Equipment for an Undergraduate Biotechnology Laboratory Program

本科生物技术实验室项目的设备

• 批准号: 8851464
• 负责人: Alfred Ordman
• 金额: --
• 依托单位: Beloit College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8851464&HistoricalAwards=false
• 关键词: Equipment; Undergraduate; Biotechnology; Laboratory; Program

Integrated biotechnology and computer labs for the Biochemistry program will be set up to train biology, biochemistry, chemistry and other majors in recombinant DNA and monoclonal antibody production and scale-up methods. Because these methods have become basic to many areas of science, should develop these skills as undergraduates for use in required internships and their own research. The number of biochemistry majors in this department has steadily increased over the past seven years; 80% of them going on to medical or graduate schools. DNA electrophoresis and blotting equipment, an inverted microscope, an ultracold freezer, a preparative centrifuge, a fermenter and two spectrophotometers permit DNA restriction mapping, cloning, and the production of proteins and monoclonal antibodies. A Biology Computing Center is being established where faculty developed software allows students to gain experience with experimental design, data collection and analysis, in addition to their lab experience. Two innovative features of this project are 1) the integration of the hands-on labs with computer simulations, allowing for practical lab experience with realistic experimental design and data analysis in a short period at low cost, and 2) the way in which labs prepare students for internships and research where people cooperate on long- term projects that integrate several methods. The grantee institution is matching the NSF award with an equal sum obtained from non-Federal sources.

生物化学专业将建立综合生物技术和计算机实验室，培养生物学、生物化学、化学等专业的重组DNA和单克隆抗体生产和放大方法。因为这些方法已经成为许多科学领域的基础，所以学生应该在本科生阶段培养这些技能，用于必要的实习和自己的研究。在过去的七年里，这个部门的生物化学专业的人数稳步增加;他们中的80%进入医学或研究生院。DNA电泳和印迹设备、倒置显微镜、超冷冷冻机、制备型离心机、发酵罐和两个生物计量器允许DNA限制性图谱、克隆以及蛋白质和单克隆抗体的生产。正在建立一个生物计算中心，教师开发的软件允许学生获得实验设计，数据收集和分析的经验，除了他们的实验室经验。该项目的两个创新特点是：1）将动手实验室与计算机模拟相结合，允许在短时间内以低成本获得实际的实验室经验，并进行逼真的实验设计和数据分析; 2）实验室为学生实习和研究做好准备的方式，人们在长期项目中合作，整合多种方法。受资助机构正在将NSF奖励与从非联邦来源获得的相等金额相匹配。