Role of the Gsb and ciD-Ce Genes in Fly Development

Gsb 和 ciD-Ce 基因在果蝇发育中的作用

• 批准号: 8901394
• 负责人: Robert Holmgren
• 金额: $ 25.71万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8901394&HistoricalAwards=false
• 关键词: Role; Gsb; ciD; Ce; Genes

Dr. Holmgren is studying the process of pattern formation within segments of Drosophila, and is particularly interested in the function of two gene clusters, gooseberry (gsb) and cubitus interruptus Dominant-Cell (ciD-Ce). He hopes to determine the role of the gsb, ciD and Ce genes in segmental pattern formation by studying their molecular function and their relationship to other segmentation genes. In his experiments,he will characterize the organization and pattern of expression of the ciD and Ce genes; make antibodies to the gsb, ciD and Ce gene products; determine the hierarchy of gene expression that leads to the formation of posterior structures within segments; and study the promoter regions of the gsb, ciD and Ce genes. By completing these experiments, he should understand how cells in the posterior part of the segment are distinguished from anterior cells. %%% Many animals are segmented. There is increasing evidence that some of the insect and vertebrate genes which control segmentation share homologies. The study of these genes in Drosophila is enhanced and facilitated by the extensive knowledge of genetics in this organism. The knowledge gained will most likely have more general relevance. %%%//

Holmgren博士正在研究果蝇节段内模式形成的过程，他特别感兴趣的是两个基因簇的功能，即醋栗(GSB)和肘状中断优势细胞(CID-CE)。他希望通过研究GSB、CID和Ce基因的分子功能以及它们与其他片段基因的关系，来确定它们在片段模式形成中的作用。在他的实验中，他将描述Cid和Ce基因的组织和表达模式；制作针对Gsb、Cid和Ce基因产物的抗体；确定导致片段内后部结构形成的基因表达层次；以及研究Gsb、Cid和Ce基因的启动子区域。通过完成这些实验，他应该了解节段后部的细胞是如何与前部细胞区分开来的。很多动物都是被分割的。越来越多的证据表明，一些控制分段的昆虫和脊椎动物基因具有同源性。这些基因在果蝇中的研究因其广泛的遗传学知识而得到加强和便利。所获得的知识很可能具有更广泛的相关性。%//