Dissertation Research: A Molecular Investigation of Higher Level Phylogeny of the Conifers

论文研究：针叶树高级系统发育的分子研究

• 批准号: 8901397
• 负责人: Leo Hickey
• 金额: $ 1.16万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-15 至 1991-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8901397&HistoricalAwards=false
• 关键词: Dissertation; Research; Molecular; Investigation; Higher

The doctoral student, Linda Raubeson, working under the supervision of Dr. Leo Hickey at Yale University, will undertake a study of the evolutionary history of the 7 families of conifers (pines), based on two kinds of molecular data, chloroplast DNA (cpDNA) structural rearrangements, and sequence data from the nuclear 18s rDNA (ribosomal) gene. The time since the 7 families became separate evolutionary lineages is estimated to be 300 million years; the chosen molecular characters are evolutionarily conservative and should be informative at that time depth. The proposed research timetable covers two years; although, a doctoral student at Yale, Raubeson will spend the first year doing the cpDNA work with Robert Jansen at the University of Connecticut and six months of the second year sequencing the 18s rDNA genes with Elizabeth Zimmer at LSU. The student has already isolated total DNA from 23 species including representatives of all 7 families and has done limited restriction enzyme digests and mapping. Zimmer has sequenced portions of the 18s rDNA gene for conifers. Both data types appear to be informative at the appropriate time depth. The completed research will clarify our understanding of the evolutionary history of this economically important assemblage of trees.

这位名为Linda Raubeson的博士生在耶鲁大学Leo Hickey博士的指导下工作，她将基于两种分子数据-叶绿体DNA(CpDNA)结构重排和核18S rDNA(核糖体)基因序列数据，对针叶树(松属)7个家族的进化史进行研究。这7个家族成为独立进化谱系的时间估计为3亿年；所选的分子特征在进化上是保守的，在那个时间深度应该是信息丰富的。拟议的研究时间表包括两年；尽管作为耶鲁的博士生，劳贝森将在第一年与康涅狄格大学的罗伯特·詹森一起进行cpDNA工作，第二年的六个月将与路易斯安那州立大学的伊丽莎白·齐默一起测序18S rDNA基因。这位学生已经从23个物种中提取了总DNA，包括所有7个科的代表物种，并进行了有限的限制性内切酶消化和图谱绘制。齐默已经对针叶树的18S rDNA基因的一部分进行了测序。在适当的时间深度，这两种数据类型似乎都是信息性的。完成的研究将澄清我们对这一具有重要经济意义的树木组合的进化史的理解。