US-Mexico Cooperative Research: cGMP Regulated Ionic Channels in Sperm

美国-墨西哥合作研究：cGMP 调节精子中的离子通道

• 批准号: 8901721
• 负责人: Bennett Shapiro
• 金额: $ 1.06万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8901721&HistoricalAwards=false
• 关键词: US; Mexico; Cooperative; Research; cGMP

This award will support Prof. Bennett Shapiro of the University of Washington in a research collaboration with Prof. Alberto Darszon of the Centro de Investigacrones y Estudios Avanzador (CINVESTAV) in Mexico City. The reserarchers intend to explore the mechanism whereby sea urchinsperm respond to eggs of the same species. It is known that peptides that diffuse from the egg to bind at the sperm surface cause the sperm to alter several ultra cellular parameters. The pepticles may play a chemotactic role and also cause transient elevations in ultracellular ıCa2+! and ıcEMP!. Preliminary data suggests the presence of a cGMP- dependent Ca2+ chfannel in plasma membranes from the sperm. Possible candidates for such a channel will be purified and their characteristics explored in reconstituted bilayer systems. Such a channel may be a component of the signal tranduction pathway of sperm chemotaxis; an analysis of its properties may clarify the mechanism of the analogoris channel that is responsible for photo transduction.l The sea urchin sperm offers some distinct advantages one more complex cell types as a basic model for chemostraxis. Many of the superflous (in this context) cellular activities related to the cell cycle, growth, and differentiation are absent from the spermitozoan. The amplified metabolism of this non-dividing cell and its limited stimulus for chemotaxis are in contrast to E. coli, which respond to a host of attractants and repellants and are actively growing and dividing. Thus the metabolically mono focused sperm array allow researchers to unearth some biochemical mechanism in the pathway of chemotaxis. By combing the strengthsof the Mexian group in planar bilayer studies with special interests in the recoinstitution of membraneprotein channels, with the experience of the U.S. group in functional studies of purified proteins that mediato cellular behavior, this study has the potential to increase our understanding of sperm chemotaxis and egg-sperm interaction.

该奖项将支持华盛顿大学的班尼特·夏皮罗教授与墨西哥城研究与研究中心（CINVESTAV）的阿尔贝托·达尔森教授的一项研究合作。研究人员打算探索海胆精子对同一物种的卵子做出反应的机制。众所周知，从卵子扩散到精子表面结合的肽会导致精子改变几个超细胞参数。这些微粒可能起到趋化作用，也会引起超细胞ıCa2+！和ıcEMP !初步数据表明，在精子的质膜中存在cGMP依赖的Ca2+通道。这种通道的可能候选物将被纯化并在重组双层系统中探索其特性。该通道可能是精子趋化性信号转导通路的一个组成部分；对其性质的分析可以阐明负责光转导的类似通道的机制。海胆精子作为化学排斥的基本模型，具有一些独特的优势，可以作为更复杂的细胞类型。许多与细胞周期、生长和分化相关的细胞活动（在这种情况下）在精子中是不存在的。这种非分裂细胞的代谢放大及其对趋化性的有限刺激与大肠杆菌相反，大肠杆菌对许多引诱剂和驱避剂作出反应，并积极生长和分裂。因此，代谢单聚焦精子阵列使研究人员能够揭示趋化途径中的一些生化机制。通过结合墨西哥小组在平面双层研究中的优势和对膜蛋白通道重组的特殊兴趣，以及美国小组在介导细胞行为的纯化蛋白的功能研究中的经验，本研究有可能增加我们对精子趋化性和卵子-精子相互作用的理解。