Molecular Genetic Investigations of Fungal Pathogenesis in Rice Blast Disease

稻瘟病真菌发病机制的分子遗传学研究

• 批准号: 8903620
• 负责人: John Hamer
• 金额: $ 25.9万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-10-15 至 1993-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8903620&HistoricalAwards=false
• 关键词: Molecular; Genetic; Investigations; Fungal; Pathogenesis

Rice blast is a devastating plant disease caused by the fungus Magnaporthe grisa. Genetic analysis with this fungal pathogen has demonstrated that numerous genetic loci are required for infecting and destroying rice plants. Because of the genetic and biochemical tractability of this fungus and its cultivated host plant, we have decided that this disease represents a unique opportunity to begin a detailed genetic and molecular analysis of fungal pathogenicity on rice by altering the shape of various fungal cells necessary for rice plant infection. We have named this genetic locus, SMO because Smo-mutations dramatically alter the shape of the asexual spores (spore morphology). In this proposal, we described our approach to cloning and analyzing the function of the SMO locus. We shall clone the SMO locus by complementation of Smo-mutants with cloned M.grisea DNA. We will prove by genetic analysis, that DNA fragments that complement the Smo-phenotype encode the SMO gene. We will sequence the wild type SMO gene and begin an analysis of the function of the SMO gene product. We will also use cloned DNA fragments to investigate the origin of Smo-mutations, which appear to occur at a high frequency. Finally we will investigate the consequences of Smo-mutations on M.grisea growth inside rice plants. The results of this research will provide new information on the formation of infection structures in plant pathogenic fungi, as well as provide insights into the mechanisms of cell shape determination in fungi.

稻瘟病是一种由真菌引起的毁灭性植物病害 稻瘟病菌 这种真菌病原体的基因分析 已经证明，许多基因位点是必需的， 感染和破坏水稻植株。 由于遗传和 该真菌及其栽培宿主的生化易处理性 植物，我们已经决定，这种疾病代表了一种独特的 有机会开始详细的遗传和分子分析， 通过改变水稻叶片的形状， 水稻植株感染所必需的真菌细胞。 我们命名 这个基因位点，SMO，因为Smo突变显着改变 无性孢子的形状（孢子形态学）。 在这 建议，我们描述了我们的方法来克隆和分析 SMO基因座的功能。我们将克隆SMO基因座， Smo突变体与克隆的稻瘟病菌DNA互补。 我们将 通过遗传分析证明，与基因互补的DNA片段 Smo-表型编码SMO基因。 我们将对野生的 型SMO基因，并开始分析SMO的功能 基因产物 我们还将使用克隆的DNA片段， 研究Smo突变的起源，这些突变似乎发生在 高频率。 最后我们会调查后果 Smo突变对稻瘟病菌在水稻植株中生长的影响。 的 这项研究的结果将提供新的信息 植物病原真菌感染结构的形成，如 并提供了细胞形状机制的见解 真菌中的测定