Cryo-Electron Microscopy and Image Analysis of Cauliflower Mosiac Virus

花椰菜花叶病毒的冷冻电子显微镜和图像分析

• 批准号: 8905062
• 负责人: Timothy Baker
• 金额: $ 11万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905062&HistoricalAwards=false
• 关键词: Cryo; Electron; Microscopy; Image; Analysis

The native three-dimensional structure of cauliflower mosaic virus (CaMV) will be studied using recently developed cryo-electron microscopy and image analysis techniques. CaMV is one of the most extensively studied plant viruses, in part, because it was the first plant virus in which a double-stranded DNA genome was discovered. However, little is known about the details of the organization and assembly of the DNA and protein components. The resulting structural model will provide new insights about the symmetry and packing arrangement of the protein subunits in the capsid and may reveal new details concerning protein-DNA interactions within the structure. CaMV is the type member of the caulimovirus family, the only group of plant viruses known to encapsidate a genome of double-stranded DNA. Caulimoviruses are unique among small DNA viruses of eukaryotes in being assembled in the cytoplasm rather than the nucleus of their host cells. They serve as a suitable model system for studying plant gene organization, expression and replication and they also are potentially useful as gene vectors for the genetic manipulation of higher plants. In addition, there are striking similarities between CaMV and certain animal viruses. For example, CaMV and the retroviruses both replicate by reverse transcription and this represents the first such example of this phenomenon in plant cells. To fully understand how CaMV functions, detailed knowledge of its structure is essential.

花椰菜花叶病毒（CaMV）的原生三维结构将使用最新开发的冷冻电子显微镜和图像分析技术进行研究。CaMV是研究最广泛的植物病毒之一，部分原因是它是第一个发现双链DNA基因组的植物病毒。然而，人们对DNA和蛋白质成分的组织和组装的细节知之甚少。由此产生的结构模型将为衣壳中蛋白质亚基的对称性和包装排列提供新的见解，并可能揭示结构中蛋白质- dna相互作用的新细节。CaMV是茎状病毒家族的类型成员，是已知的唯一一组能够封装双链DNA基因组的植物病毒。在真核生物的小DNA病毒中，茎状病毒是独特的，它在细胞质中而不是在宿主细胞的细胞核中组装。它们为研究植物基因的组织、表达和复制提供了合适的模型系统，同时也为高等植物的遗传操作提供了潜在的基因载体。此外，CaMV与某些动物病毒有惊人的相似之处。例如，CaMV和逆转录病毒都通过逆转录进行复制，这是植物细胞中这种现象的第一个例子。要充分了解CaMV的功能，详细了解其结构是必不可少的。