Developmental Physiology of Dorsal-Ventral Currents During Oogenesis

卵子发生过程中背腹电流的发育生理学

• 批准号: 8905552
• 负责人: Joseph Kunkel
• 金额: $ 8.7万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905552&HistoricalAwards=false
• 关键词: Developmental; Physiology; Dorsal; Ventral; Currents

The stable currents which have been demonstrated to enter the ventral and leave the dorsal surface of the vitellogenic oocyte of Blattella germanica are a very early and a very striking demonstration of dorsal ventral polarity. They may represent the earliest physiological process to be directly correlated with the establishment of a dorsal ventral polarity. To elucidate the ionic basis of these currents Dr. Kunkel plans to use ionic replacement, channel blocking and metabolic poisons to affect the pattern of measurable currents. The effects of natural serum components present during vitellogenesis on the currents will also be examined, including vitellogenin, other serum proteins and juvenile hormone. The effects of removal of the follicle epithelium on the currents will be determined. The physiology of these currents will be investigated using the vibrating probe and patch clamping. This study will allow Dr. Kunkel to examine the origin and physiological basis of dorsal ventral polarity. %%% The establishment of asymmetry is an early and fundamental step in the development of an organism. It occurs before fertilization. The unfertilized egg already shows polarity. Dr. Kunkel is investigating the establishment of this polarity early in the development of the egg.

已被证明进入的稳定电流 腹侧和离开卵黄发生卵母细胞的背面 德国小蠊的出现非常早，也非常引人注目 背腹极性的证明。他们可能代表 最早的生理过程直接与 建立背腹极性。阐明本 这些电流的离子基础Kunkel博士计划使用离子 替代，通道阻断和代谢毒物，以影响 可测量电流的模式。天然血清的作用 在卵黄发生过程中，电流中存在的成分将 还可以检查，包括卵黄蛋白原，其他血清蛋白 和保幼激素去除卵泡的效果 将确定上皮对电流的影响。的生理学 将使用振动探针研究这些电流， 膜片钳这项研究将允许Kunkel博士检查 背腹极性的起源和生理基础。 %%% 建立不对称性是一个早期和根本性的步骤 在有机体的发育过程中。它发生在 受精未受精的卵子已经显示出极性。博士 Kunkel正在研究这种极性的早期建立 在卵子的发育过程中。