Alteration of Ectomycorrhizal Fungus Community Structure: A DNA Fingerprinting Approach

外生菌根真菌群落结构的改变：DNA 指纹分析方法

• 批准号: 8906666
• 负责人: Robert Fogel
• 金额: $ 7.5万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8906666&HistoricalAwards=false
• 关键词: Alteration; Ectomycorrhizal; Fungus; Community; Structure

The objectives of the proposed research is to use polymerase chain reaction amplification of DNA (pcr) in conjunction with base sequencing to identify ectomycorrhizal fungi competing for colonization sites on the root systems of white pine seedlings. At present, only two or three ectomycorrhizal fungi can be identified on the basis of ectomycorrhiza morphology since the taxonomy of these fungi is based on characteristics of the reproductive structures, e.g., mushrooms, truffles. A reliable means of identifying fungi from ectomycorrhizae and hyphae is needed if the importance of fungi in major ecosystem processes, e.g. decomposition, nutrient absorption of trees, alteration of community composition by pathogenic fungi, etc., is to be determined. Although the fungi to be studied are native to the area and not genetically engineered, the results of introducing a known fungus into the soil and verifying recovery of the same fungus by DNA fingerprinting will be directly applicable to experiments introducing genetically engineered microbes.

这项研究的目的是利用聚合酶 DNA链反应扩增（PCR）结合 碱基测序，以确定外生菌根真菌竞争， 在白松幼苗根系上的定殖位点。 目前，只有两种或三种外生菌根真菌可以 根据外生菌根形态学鉴定， 这些真菌的分类是基于特征的 生殖结构，例如，蘑菇松露 一个可靠 从外生菌根和菌丝中鉴定真菌的方法是 如果真菌在主要生态系统过程中的重要性， 例如分解、树木的养分吸收、 病原真菌的群落组成等，要 测定 虽然要研究的真菌是原产于 地区，而不是基因工程，结果引入了一个 已知真菌进入土壤，并验证其回收率 真菌DNA指纹将直接适用于 引入基因工程微生物的实验。