Acquisition of an Isotope Ratio Mass Spectrometer for Analysis of 15n/13C Enriched Biological Materials and Development of 15N Trace Gas Analysis

购置同位素比质谱仪用于分析 15n/13C 富集生物材料并开发 15N 痕量气体分析

• 批准号: 8907131
• 负责人: Mary Firestone
• 金额: $ 11.92万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8907131&HistoricalAwards=false
• 关键词: Acquisition; Isotope; Ratio; Mass; Spectrometer

This proposal includes both the purchase of an instrument and the development of a new analytical capability for direct analysis of 15N gases. A primary group of four investigators at the University of California, Berkeley and Stanford University Stanford proposes to acquire an isotope ratio mass spectrometer with a direct combustion unit for automated analysis of 15N-and 13C-enriched materials. The research projects in which the stable isotope analysis will be used include terrestrial nutrient cycling, soil microbial ecology, plant physiological ecology, trace gas interactions between soil and atmosphere, and analysis of metabolic pathways. The immediate availability to these research programs of this recently developed, high sample capacity, small sample size, stable isotope/total N and C analysis system will provide a powerful analytical capacity for routine application in a number of very active ecology and physiology research programs in Northern California. The development of the mass spectrometer for direct quantification of 15N N20, N2, and NO (separated by capillary chromatographic methods) will facilitate major new research investigations in the origin and control of trace nitrogen gases involved in global ozone depletion and climatic change.

这一建议包括购买一种工具， 发展新的分析能力， 15 N气体分析 一个由四名研究人员组成的主要小组， 加州大学伯克利分校和斯坦福大学 斯坦福大学提议购买同位素比质谱仪 带有直接燃烧装置，用于自动分析15 N-和 富含13 C的材料。 研究项目中， 将使用稳定同位素分析，包括陆地营养物 循环，土壤微生物生态，植物生理生态， 土壤与大气之间的痕量气体相互作用及其分析 代谢途径。 立即提供这些 最近开发的高样本研究项目 容量、小样本量、稳定同位素/总氮和总碳 分析系统将提供强大的分析能力， 在一些非常活跃的生态系统中的常规应用， 北方加州的生理学研究项目。 的 质谱仪的研制 15 N N2 O、N2和NO（通过毛细管色谱法分离 方法）将促进重大新的研究调查， 全球大气中微量氮的来源与控制 臭氧消耗和气候变化。