RUI: Acquisition of an Ultramicratome and Acillaries for Research in the Department of Biology

RUI：购买超微切片机和辅助设备用于生物系研究

• 批准号: 8907860
• 负责人: Nathan Okia
• 金额: $ 2万
• 依托单位: Auburn University at Montgomery
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8907860&HistoricalAwards=false
• 关键词: RUI; Acquisition; Ultramicratome; Acillaries; Research

A proposal is being submitted to acquire an ultramicrotome, an anti-vibration table and a knife-maker. There are four proposed projects submitted. Project one will study the fine structure of the skink (lizard) spermatozoan and its development. Its principle aim will be to elucidate the fine structure of the centriolar region (between head and tail) of the lizard sperm and to describe the process of spermatogenesis in the southern united states skinks of the genus Eumeces. Project two is aimed at elucidating the details of hormone-receptor binding between gonadotrophins (LH) and the mouse epididymis at the ultrastructural level. Results so far obtained at the light microscope level indicate a possible concentration of receptors for LH in the mouse epididymis perhaps in the Golgi complex and endoplasmic reticulum. The actual site of binding may be determined using electron microscope immunohistochemistry. Project three will study fungus-host interactions between fungal endophytes of the family Clavipitaceae (Ascomycotina) and their grass hosts with the objective of unraveling the fine structural changes of fungal and host cells that permit the two organisms to exist mutualistically. The fourth project will examine several isolates of Haemophilus somnus, a bacterium that is the causative agent of thromboembolic meningoencephalitis and certain types of reproductive failure in cattle, for possible presence of a capsule, with the objective of discovering its method of adherence to host cells.

一份收购超微切割机、防振台和制刀机的提案正在提交中。提交了四个拟议的项目。项目一将研究蜥蜴精子的精细结构及其发育。它的主要目的将是阐明蜥蜴精子中心粒区域(头部和尾部之间)的精细结构，并描述美国南部石龙属的精子发生过程。项目二旨在从超微结构水平阐明促性腺激素与小鼠附睾之间激素受体结合的细节。到目前为止，在光学显微镜水平上获得的结果表明，小鼠附睾体中可能存在促黄体激素受体，可能存在于高尔基复合体和内质网中。结合的实际位置可以用电子显微镜免疫组织化学来确定。项目三将研究橡胶科真菌内生菌(子囊菌亚门)和它们的草寄主之间的真菌-寄主相互作用，目的是揭示真菌和寄主细胞的精细结构变化，使这两种生物能够共生。第四个项目将检查睡眠嗜血杆菌的几个分离株，这是一种导致牛血栓栓塞性脑膜脑炎和某些类型繁殖失败的细菌，以检查是否存在胶囊，目的是发现其与宿主细胞的黏附方法。