Use of In Situ Hybridization to Study Regulation of LHRU Neuronal Activity

利用原位杂交研究 LHRU 神经元活性的调节

• 批准号: 8910024
• 负责人: Sandra Petersen
• 金额: $ 1.2万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1990-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8910024&HistoricalAwards=false
• 关键词: Use; Situ; Hybridization; Study; Regulation

Dr Petersen has received a research planning grant to refine and validate a new technique which promises to be useful for measuring the activity of individual neurons involved in the control of behavior and pituitary function. Using the in situ hybridization technique, she will be able to determine basic mechanisms by which gonadal steroid hormones can affect anatomically diverse populations of peptidergic neurons in the brain. Specifically, Dr. Peterson will examine the activity of luteinizing hormone-releasing hormone (LHRH). Rising plasma estrogen concentrations from maturing ovarian follicles trigger an increase in LHRH which is released into the pituitary portal circulation. The rise in LHRH is critical for release of pituitary hormones and subsequently, ovulation. Using this state-of-the-art technique, Dr. Peterson will identify estrogen- sensitive neuronal systems which activate LHRH neurons. This information can be used to help understand how neurons making the same product can control different brain functions involved in reproduction and other hormone-regulated physiological functions.

彼得森博士已收到研究规划补助金，以完善和 验证一项有望对以下方面有用的新技术 测量参与神经元活动的单个神经元的活动， 控制行为和垂体功能。 同步原位 杂交技术，她将能够确定基本的 性腺类固醇激素影响 解剖学上不同的肽能神经元群体， 个脑袋 具体来说，彼得森博士将研究的活动， 促黄体生成激素释放激素（LHRH）。 上升血浆 成熟卵泡中的雌激素浓度 释放到垂体门脉的LHRH增加 流通 LHRH的升高对于释放 垂体激素和随后的排卵 使用此 彼得森医生会用最先进的技术鉴定雌激素 敏感的神经系统，激活LHRH神经元。 这 这些信息可以用来帮助理解神经元是如何制造 同一种产品可以控制不同的大脑功能， 生殖和其他由植物调节的生理功能。