Core Facility for Macromolecular Sequencing, Synthesis, and Analysis

该奖项为公共卫生研究所提供资金，用于购买或升级合成或分析蛋白质所需的各种仪器，以及使用聚合酶链反应合成DNA所需的热循环器。这些设备将用于帮助提供一个核心设施，为研究各种生物问题的研究人员提供服务。这些包括DNA转化和位点特异性重组过程中DNA摄取的机制，丝状病毒病毒粒子中蛋白质-核酸相互作用的作用，酵母中质子易位的机制，细菌中孢子形成的调节以及许多其他问题。我们对各种生物过程的理解的迅速发展，在很大程度上是由于用于合成、纯化和分析蛋白质的精密和高灵敏度仪器的发展。通过该奖项购买的设备将使一组可靠的研究人员能够从事与蛋白质结构和功能有关的各种项目。他们的进展速度将大大提高装备的核心设施，使之成为可能通过这个奖项。

This award provides funds to the Public Health Research Institute for the purchase or upgrade of a variety of instruments needed for the synthesis or analysis of proteins, and for a thermal cycler for synthesis of DNA using the polymerase chain reaction. The equipment will be used to help furnish a core facility that serves investigators involved in studies of a wide variety of biological problems. These include the mechanism of DNA uptake during DNA transformation and of site specific recombination, the role of protein-nucleic acid interactions in the virions of filamentous viruses, the mechanism of proton translocation in yeast, the regulation of sporulation in bacteria, and a number of other problems. The rapid advance of our understanding of a variety of biological processes has been due in no small part to the development of sophisticated and highly sensitive instruments for the synthesis, purification and analysis of proteins. The equipment purchased through this award will enable a group of solid investigators to pursue a variety of projects concerned with protein structure and function. Their rates of progress will be substantially augmented by the equipping of a core facility made possible through this award.