Localization of Activated cAMP-Dependent Protein Kinase

该项目的长期目标是阐明肽激素结合后camp依赖性蛋白激酶的激活如何在细胞中引起反应。具体来说，camp依赖性蛋白激酶在Y-1（一种肾上腺肿瘤细胞系）和牛肾上腺皮质细胞的类固醇生成和细胞增殖中的作用将被研究。在这项研究中，一个特定的和直接的细胞化学，荧光，光镜探针和免疫金电镜程序来定位自由催化和调节亚基的亚细胞位点将与类固醇分泌和细胞增殖的生化分析相关。本研究结果将有助于了解camp依赖性蛋白激酶在肾上腺皮质细胞触发类固醇激素产生和细胞增殖中的生理作用。

The long term goal of this project is to elucidate how the activation of cAMP-dependent protein kinase following peptide hormone binding brings about a response in the cell. Specifically, the role of cAMP-dependent protein kinase in steroidogenesis and cell proliferation in Y-1, an adrenal tumor cell line, and bovine adrenal cortical cells will be studied. In this study, a specific and direct cytochemical, fluorescent, light microscopic probe and an immunogold electron microscopic procedure for localizing the subcellular sites of free catalytic and regulatory subunits will be correlated to biochemical assays of steroid secretion and cell proliferation. The results of this research will contribute to basic knowledge of the physiological role of cAMP-dependent protein kinase in triggering steroid hormone production and cell proliferation by adrenal cortical cells.